TY - JOUR
T1 - Identification and Functional Characterization of Fungal Chalcone Synthase and Chalcone Isomerase
AU - Furumura, Sho
AU - Ozaki, Taro
AU - Sugawara, Akihiro
AU - Morishita, Yohei
AU - Tsukada, Kento
AU - Ikuta, Tatsuya
AU - Inoue, Asuka
AU - Asai, Teigo
N1 - Funding Information:
We thank Prof. Katsuya Gomi (Tohoku University) and Prof. Katsuhiko Kitamoto (The University of Tokyo) for providing the expression vectors and the fungal strain, Prof. Hideaki Oikawa (Hokkaido University), Prof. Atsushi Minami (Hokkaido University), and Prof. Jun-ichi Maruyama (The University of Tokyo) for the genome editing system of A. oryzae, Prof. Ikuro Abe (The University of Tokyo) and Prof. Takayoshi Awakawa (The University of Tokyo) for providing the protein expression vector, and Prof. Tomoyoshi Akashi (Nihon University) for the CHI expression plasmid. We also thank Yuto Homma (Tohoku University) for his help in bioinformatic analysis of A domains. Molecular docking was performed on the NIG supercomputer at ROIS National Institute of Genetics. This work was supported by JSPS KAKENHI (22H02775 (T.A.), 22K19095 (T.O.), 21H04791 (A.I.), and 21H05113 (A.I.), AMED (20wm0325003 (T.A.) and 22gm1610007 (T.A.)), JST FOREST Program (JPMJFR205W (T.A.) and JPMJFR215T (A.I.)), and Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED (JP22am121038 (T.A.) and JP22ama121040 (T.O.)).
Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society and American Society of Pharmacognosy.
PY - 2023/2/24
Y1 - 2023/2/24
N2 - By mining fungal genomic information, a noncanonical iterative type I PKS fused with an N-terminal adenylation-thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain was involved in the Claisen-type cyclization. An enzyme responsible for formation of (2S)-flavanone in the biosynthesis of fungal flavonoids was also identified. Collectively, these findings demonstrate unprecedented fungal biosynthetic machinery leading to plant-like metabolites.
AB - By mining fungal genomic information, a noncanonical iterative type I PKS fused with an N-terminal adenylation-thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain was involved in the Claisen-type cyclization. An enzyme responsible for formation of (2S)-flavanone in the biosynthesis of fungal flavonoids was also identified. Collectively, these findings demonstrate unprecedented fungal biosynthetic machinery leading to plant-like metabolites.
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U2 - 10.1021/acs.jnatprod.2c01027
DO - 10.1021/acs.jnatprod.2c01027
M3 - Article
C2 - 36762727
AN - SCOPUS:85147985462
SN - 0163-3864
VL - 86
SP - 398
EP - 405
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 2
ER -