TY - JOUR
T1 - Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer
AU - Yamakawa, Hiromitsu
AU - Nagase, Satoru
AU - Yuki, Michihiro
AU - Shiwaku, Hiromi O.
AU - Furukawa, Toru
AU - Yoshinaga, Kousuke
AU - Soeda, Eiichi
AU - Hoshi, Masato
AU - Hayashi, Yutaka
AU - Sato, Shinji
AU - Yajima, Akira
AU - Horii, Akira
PY - 1998/9
Y1 - 1998/9
N2 - In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two-color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which Notl, Sail, Sinal, and Xhol were clustered, suggesting the possible existence of a CpG island.
AB - In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two-color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which Notl, Sail, Sinal, and Xhol were clustered, suggesting the possible existence of a CpG island.
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U2 - 10.1002/(SICI)1098-2264(199809)23:1<74::AID-GCC12>3.0.CO;2-E
DO - 10.1002/(SICI)1098-2264(199809)23:1<74::AID-GCC12>3.0.CO;2-E
M3 - Article
C2 - 9714001
AN - SCOPUS:0031948962
SN - 1045-2257
VL - 23
SP - 74
EP - 77
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 1
ER -
