TY - JOUR
T1 - Identification of a novel arsenic resistance transposon nested in a mercury resistance transposon of bacillus sp. Mb24
AU - Chien, Mei Fang
AU - Ho, Ying Ning
AU - Yang, Hui Erh
AU - Narita, Masaru
AU - Miyauchi, Keisuke
AU - Endo, Ginro
AU - Huang, Chieh Chen
N1 - Funding Information:
This research was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Funding: This research was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Number 19H02654 (Grant-in-Aid for Scientific Research(B)). Part of this research was supported by (JSPS) Grant Number 19H02654 (Grant-in-Aid for Scientific Research(B)). Part of this research was supported by (JSPS) KAKENHI KAKENHI Grant Grant Number Number 18F18393 18F18393 (Grant-in-Aid (Grant-in-Aid for for JSPS JSPS Research Fellow). We appreciate Fan-Fan Chen from National Chung Hsing University, Taichung, Taiwan and Kohei Obata from Tohoku Gakuin University, Miyagi, Japan for their experimental support in this study.
Funding Information:
Author Contributions: M.-F.C.; data curation, methodology, investigation, project administration, validation, AuthovrisCuaolniztaritibount,iwonrist:inMg-. -oFr.iCgi.n; adla dtaracfut prarteipoanr,amtioenthw,orditoinlgo-grye,vinievwes& ti egdaittiionng,, fpurnodjeinctgaadcqmuiisniitsiotrna,tYio.-nN,.Hva.;liddaattaion, visuacliuzraattioionn,, wmreittihondgo—loogryig, iinnavlsetdigraafttiopnr,e vpaalidraattiioonn,, vwisruitailnizga—tiornev, wierwiti&nge-doirtiignign,alfudnradfitn pgreapcaqruaitsiiotnio,wn,riYti.n-Ng-.H.; review & editing, H.-E.Y.; data curation, investigation, M.N.; methodology, data curation, investigation, validation K.M; methodology, data curation, investigation, validation, G.E, and C.-C.H.; project administration, validationwritingK.M.;-reviemweth& eoddoitloinggy,,sudatapervision,curation,fundinvestigation,ing acquisition.validation, G.E., and C.-C.H.; project administration, writing—review & editing, supervision, funding acquisition. Funding: This research was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Funding: This research was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Number 19H02654 (Grant-in-Aid for Scientific Research(B)). Part of this research was supported by (JSPS) Grant Number 19H02654 (Grant-in-Aid for Scientific Research(B)). Part of this research was supported by (JSPS) KAKENHI Grant Number 18F18393 (Grant-in-Aid for JSPS Research Fellow).
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/11
Y1 - 2019/11
N2 - A novel TnMERI1-like transposon designated as TnMARS1 was identified from mercury resistant Bacilli isolated from Minamata Bay sediment. Two adjacent ars operon-like gene clusters, ars1 and ars2, flanked by a pair of 78-bp inverted repeat sequences, which resulted in a 13.8-kbp transposon-like fragment, were found to be sandwiched between two transposable genes of the TnMERI1-like transposon of a mercury resistant bacterium, Bacillus sp. MB24. The presence of a single transcription start site in each cluster determined by 5′-RACE suggested that both are operons. Quantitative real time RT-PCR showed that the transcription of the arsR genes contained in each operon was induced by arsenite, while arsR2 responded to arsenite more sensitively and strikingly than arsR1 did. Further, arsenic resistance complementary experiments showed that the ars2 operon conferred arsenate and arsenite resistance to an arsB-knocked out Bacillus host, while the ars1 operon only raised arsenite resistance slightly. This transposon nested in TnMARS1 was designated as TnARS1. Multi-gene cluster blast against bacteria and Bacilli whole genome sequence databases suggested that TnMARS1 is the first case of a TnMERI1-like transposon combined with an arsenic resistance transposon. The findings of this study suggested that TnMERI1-like transposons could recruit other mobile elements into its genetic structure, and subsequently cause horizontal dissemination of both mercury and arsenic resistances among Bacilli in Minamata Bay.
AB - A novel TnMERI1-like transposon designated as TnMARS1 was identified from mercury resistant Bacilli isolated from Minamata Bay sediment. Two adjacent ars operon-like gene clusters, ars1 and ars2, flanked by a pair of 78-bp inverted repeat sequences, which resulted in a 13.8-kbp transposon-like fragment, were found to be sandwiched between two transposable genes of the TnMERI1-like transposon of a mercury resistant bacterium, Bacillus sp. MB24. The presence of a single transcription start site in each cluster determined by 5′-RACE suggested that both are operons. Quantitative real time RT-PCR showed that the transcription of the arsR genes contained in each operon was induced by arsenite, while arsR2 responded to arsenite more sensitively and strikingly than arsR1 did. Further, arsenic resistance complementary experiments showed that the ars2 operon conferred arsenate and arsenite resistance to an arsB-knocked out Bacillus host, while the ars1 operon only raised arsenite resistance slightly. This transposon nested in TnMARS1 was designated as TnARS1. Multi-gene cluster blast against bacteria and Bacilli whole genome sequence databases suggested that TnMARS1 is the first case of a TnMERI1-like transposon combined with an arsenic resistance transposon. The findings of this study suggested that TnMERI1-like transposons could recruit other mobile elements into its genetic structure, and subsequently cause horizontal dissemination of both mercury and arsenic resistances among Bacilli in Minamata Bay.
KW - Arsenic resistance operon
KW - Class II transposon
KW - Genome mining
KW - Horizontal gene transfer
KW - Mercury resistance operon
UR - http://www.scopus.com/inward/record.url?scp=85075136774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075136774&partnerID=8YFLogxK
U2 - 10.3390/microorganisms7110566
DO - 10.3390/microorganisms7110566
M3 - Article
AN - SCOPUS:85075136774
SN - 2076-2607
VL - 7
JO - Microorganisms
JF - Microorganisms
IS - 11
M1 - 566
ER -