TY - JOUR
T1 - Identification of a novel isoform of microphthalmia-associated transcription factor that is enriched in retinal pigment epithelium
AU - Amae, Shintaro
AU - Fuse, Nobuo
AU - Yasumoto, Ken Ichi
AU - Sato, Shigeru
AU - Yajima, Ichiro
AU - Yamamoto, Hiroaki
AU - Udono, Tetsuo
AU - Durlu, Yusuf K.
AU - Tamai, Makoto
AU - Takahashi, Kazuhiro
AU - Shibahara, Shigeki
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Encouragement of Young Scientist (to K. Y.), Scienti®c Research on Priority Areas (to K. Y.), Exploratory Research, and for Scienti®c Research (B) from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 1998/6/29
Y1 - 1998/6/29
N2 - Mutations at the mouse locus encoding microphthalmia-associated transcription factor (Mitf) affect the development of many cell types, including retinal pigment epithelium (RPE), melanocytes, mast cells, and osteoclasts. Here we have identified a novel Mitf isoform, Mitf-a, and its human homologue MITF-A by cDNA cloning. MITF-A consists of 520 amino acid residues and differs in the amino-terminus from authentic melanocyte-type MITF (MITF-M). MITF-A mRNA is widely expressed and represents a predominant MITF isoform in cultured RPE cells, whereas MITF-M mRNA is exclusively expressed in melanocytes and melanoma cells. In situ hybridization analysis suggested that Mitf-a mRNA is enriched in the prospective RPE of mouse embryo. Moreover, transient cotransfection assays suggested that MITF-A activated transcription of the tyrosinase and tyrosinase-related protein 1 genes. MITF-A/Mitf-a therefore may play an important role in melanogenesis in RPE.
AB - Mutations at the mouse locus encoding microphthalmia-associated transcription factor (Mitf) affect the development of many cell types, including retinal pigment epithelium (RPE), melanocytes, mast cells, and osteoclasts. Here we have identified a novel Mitf isoform, Mitf-a, and its human homologue MITF-A by cDNA cloning. MITF-A consists of 520 amino acid residues and differs in the amino-terminus from authentic melanocyte-type MITF (MITF-M). MITF-A mRNA is widely expressed and represents a predominant MITF isoform in cultured RPE cells, whereas MITF-M mRNA is exclusively expressed in melanocytes and melanoma cells. In situ hybridization analysis suggested that Mitf-a mRNA is enriched in the prospective RPE of mouse embryo. Moreover, transient cotransfection assays suggested that MITF-A activated transcription of the tyrosinase and tyrosinase-related protein 1 genes. MITF-A/Mitf-a therefore may play an important role in melanogenesis in RPE.
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U2 - 10.1006/bbrc.1998.8838
DO - 10.1006/bbrc.1998.8838
M3 - Article
C2 - 9647758
AN - SCOPUS:0032577909
SN - 0006-291X
VL - 247
SP - 710
EP - 715
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -