Identification of annexin 1 as a PU.1 target gene in leukemia cells

Yuko Iseki; Akemi Imoto; Toshio Okazaki; Hideo Harigae; Shinichiro Takahashi

doi:10.1016/j.leukres.2009.04.010

Identification of annexin 1 as a PU.1 target gene in leukemia cells

Yuko Iseki, Akemi Imoto, Toshio Okazaki, Hideo Harigae, Shinichiro Takahashi

HOSP - University Hospital (not affiliated with any section)

Kitasato University

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

To identify PU.1 downstream target genes, we first established PU.1-knockdown K562 (K562PU.1KD) cells expressing reduced levels of PU.1 by stably transfected PU.1 siRNAs. From microarray analysis, we found that several genes including annexin 1 were markedly induced in K562PU.1KD cells. Annexin 1 is a calcium- and phospholipid-binding protein and increased expression leads to the constitutive activation of extracellular signal-regulated kinase (ERK). Consistent with this, we observed constitutive activation of ERK in K562PU.1KD cells. Furthermore, we revealed the mRNA expression of annexin 1 was negatively correlated with PU.1 mRNA expression in 43 primary AML specimens (R = -0.31, p < 0.042).

Original language	English
Pages (from-to)	1658-1663
Number of pages	6
Journal	Leukemia Research
Volume	33
Issue number	12
DOIs	https://doi.org/10.1016/j.leukres.2009.04.010
Publication status	Published - 2009 Dec

Keywords

AML
Annexin 1
PU.1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.leukres.2009.04.010

Cite this

@article{9a6ddf07e0d34959bc7fcd5466cb5e65,

title = "Identification of annexin 1 as a PU.1 target gene in leukemia cells",

abstract = "To identify PU.1 downstream target genes, we first established PU.1-knockdown K562 (K562PU.1KD) cells expressing reduced levels of PU.1 by stably transfected PU.1 siRNAs. From microarray analysis, we found that several genes including annexin 1 were markedly induced in K562PU.1KD cells. Annexin 1 is a calcium- and phospholipid-binding protein and increased expression leads to the constitutive activation of extracellular signal-regulated kinase (ERK). Consistent with this, we observed constitutive activation of ERK in K562PU.1KD cells. Furthermore, we revealed the mRNA expression of annexin 1 was negatively correlated with PU.1 mRNA expression in 43 primary AML specimens (R = -0.31, p < 0.042).",

keywords = "AML, Annexin 1, PU.1",

author = "Yuko Iseki and Akemi Imoto and Toshio Okazaki and Hideo Harigae and Shinichiro Takahashi",

note = "Funding Information: We thank for Ms. Asami Takahashi for flow cytometric analysis and Dr. Mitsue Inomata for establishing PU.1 over-expressing cell lines. This work is supported in part by a grant from Japan Leukemia Research Foundation, Foundation from Kitasato University School of Allied Health Sciences (Grant-in-Aid for Research Project, No. 2007-1003, No. 2008-1001) and Foundation from Kitasato University Graduate School of Medical Sciences Research Project. ",

year = "2009",

month = dec,

doi = "10.1016/j.leukres.2009.04.010",

language = "English",

volume = "33",

pages = "1658--1663",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Ltd.",

number = "12",

}

TY - JOUR

T1 - Identification of annexin 1 as a PU.1 target gene in leukemia cells

AU - Iseki, Yuko

AU - Imoto, Akemi

AU - Okazaki, Toshio

AU - Harigae, Hideo

AU - Takahashi, Shinichiro

N1 - Funding Information: We thank for Ms. Asami Takahashi for flow cytometric analysis and Dr. Mitsue Inomata for establishing PU.1 over-expressing cell lines. This work is supported in part by a grant from Japan Leukemia Research Foundation, Foundation from Kitasato University School of Allied Health Sciences (Grant-in-Aid for Research Project, No. 2007-1003, No. 2008-1001) and Foundation from Kitasato University Graduate School of Medical Sciences Research Project.

PY - 2009/12

Y1 - 2009/12

N2 - To identify PU.1 downstream target genes, we first established PU.1-knockdown K562 (K562PU.1KD) cells expressing reduced levels of PU.1 by stably transfected PU.1 siRNAs. From microarray analysis, we found that several genes including annexin 1 were markedly induced in K562PU.1KD cells. Annexin 1 is a calcium- and phospholipid-binding protein and increased expression leads to the constitutive activation of extracellular signal-regulated kinase (ERK). Consistent with this, we observed constitutive activation of ERK in K562PU.1KD cells. Furthermore, we revealed the mRNA expression of annexin 1 was negatively correlated with PU.1 mRNA expression in 43 primary AML specimens (R = -0.31, p < 0.042).

AB - To identify PU.1 downstream target genes, we first established PU.1-knockdown K562 (K562PU.1KD) cells expressing reduced levels of PU.1 by stably transfected PU.1 siRNAs. From microarray analysis, we found that several genes including annexin 1 were markedly induced in K562PU.1KD cells. Annexin 1 is a calcium- and phospholipid-binding protein and increased expression leads to the constitutive activation of extracellular signal-regulated kinase (ERK). Consistent with this, we observed constitutive activation of ERK in K562PU.1KD cells. Furthermore, we revealed the mRNA expression of annexin 1 was negatively correlated with PU.1 mRNA expression in 43 primary AML specimens (R = -0.31, p < 0.042).

KW - AML

KW - Annexin 1

KW - PU.1

UR - http://www.scopus.com/inward/record.url?scp=70249109376&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70249109376&partnerID=8YFLogxK

U2 - 10.1016/j.leukres.2009.04.010

DO - 10.1016/j.leukres.2009.04.010

M3 - Article

C2 - 19428102

AN - SCOPUS:70249109376

SN - 0145-2126

VL - 33

SP - 1658

EP - 1663

JO - Leukemia Research

JF - Leukemia Research

IS - 12

ER -

Identification of annexin 1 as a PU.1 target gene in leukemia cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this