TY - JOUR
T1 - Identification of genes regulating colorectal carcinogenesis by using the algorithm for diagnosing malignant state method
AU - Ichikawa, Yasushi
AU - Ishikawa, Takashi
AU - Takahashi, Shinji
AU - Hamaguchi, Youhei
AU - Morita, Tomoyuki
AU - Nishizuka, Itaru
AU - Yamaguchi, Shigeki
AU - Endo, Itaru
AU - Ike, Hideyuki
AU - Togo, Shinji
AU - Oki, Shigeo
AU - Shimada, Hiroshi
AU - Kadota, Koji
AU - Nakamura, Shugo
AU - Goto, Hitoshi
AU - Nitanda, Hiroyuki
AU - Satomi, Susumu
AU - Sakai, Takehito
AU - Narita, Ichiei
AU - Gejyo, Fumitake
AU - Tomaru, Yasuhiro
AU - Shimizu, Kentaro
AU - Hayashizaki, Yoshihide
AU - Okazaki, Yasushi
N1 - Funding Information:
This study has been supported by a Research Grant for the RIKEN Genome Exploration Research Project from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government and ACT-JST (Research and Development for Applying Advanced Computational Science and Technology) of Japan Science and Technology Corporation (JST) to Y. Hayashizaki and by Special Coordination Funds for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government to Y. Okazaki. We thank N. Goto, M. Gariboldi, L. Marchioni, T. Kasukawa, H. Bono for technical assistance and helpful discussion.
PY - 2002
Y1 - 2002
N2 - We studied the expression profiles of various stages of colorectal tumors (adenoma (AD), seven samples; carcinoma (CA), 16 samples) by using cDNA microarrays and developed ADMS (algorithm for diagnosing malignant state) method, selecting 335 clones characteristic of CA state. We, then, applied ADMS to 12 additional samples (five from primary lesions with metastasis and seven metastases); all 16 CAs and 12 metastatic tumors were diagnosed correctly as cancerous states. Although three of the seven ADs were diagnosed as "cancerous," the large size of two of these tumors suggested their potential malignancy. Our strategy for selecting clones characteristic of the malignant state is widely applicable to diagnosis and for predicting the stage of progression during multistep carcinogenesis. Of the 335 clones we selected, 135 were known genes. Included in the 135 genes were tumor suppressor and growth factor-related genes and were consistent with the literature. ADMS is a reliable means for identifying genes useful for the diagnosis of cancer.
AB - We studied the expression profiles of various stages of colorectal tumors (adenoma (AD), seven samples; carcinoma (CA), 16 samples) by using cDNA microarrays and developed ADMS (algorithm for diagnosing malignant state) method, selecting 335 clones characteristic of CA state. We, then, applied ADMS to 12 additional samples (five from primary lesions with metastasis and seven metastases); all 16 CAs and 12 metastatic tumors were diagnosed correctly as cancerous states. Although three of the seven ADs were diagnosed as "cancerous," the large size of two of these tumors suggested their potential malignancy. Our strategy for selecting clones characteristic of the malignant state is widely applicable to diagnosis and for predicting the stage of progression during multistep carcinogenesis. Of the 335 clones we selected, 135 were known genes. Included in the 135 genes were tumor suppressor and growth factor-related genes and were consistent with the literature. ADMS is a reliable means for identifying genes useful for the diagnosis of cancer.
KW - Colorectal cancer
KW - DNA microarray
KW - Gene expression profiling
KW - Malignancy diagnosis
KW - Molecular classification
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U2 - 10.1016/S0006-291X(02)00732-5
DO - 10.1016/S0006-291X(02)00732-5
M3 - Article
C2 - 12163047
AN - SCOPUS:18644362082
SN - 0006-291X
VL - 296
SP - 497
EP - 506
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
