Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry

Tatsuya Sugawara; Jingjing Duan; Kazuhiko Aida; Tsuyoshi Tsuduki; Takashi Hirata

doi:10.1007/s11745-010-3417-0

Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry

Tatsuya Sugawara, Jingjing Duan, Kazuhiko Aida, Tsuyoshi Tsuduki, Takashi Hirata

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants.

Original language	English
Pages (from-to)	451-455
Number of pages	5
Journal	Lipids
Volume	45
Issue number	5
DOIs	https://doi.org/10.1007/s11745-010-3417-0
Publication status	Published - 2010 May

Keywords

Glycosphingolipids
HPLC (High performance liquid chromatography)
Mass spectrometry (MS)
Plant lipids
Sphingoid bases

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Cell Biology

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title = "Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry",

abstract = "We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants.",

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author = "Tatsuya Sugawara and Jingjing Duan and Kazuhiko Aida and Tsuyoshi Tsuduki and Takashi Hirata",

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T1 - Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry

AU - Sugawara, Tatsuya

AU - Duan, Jingjing

AU - Aida, Kazuhiko

AU - Tsuduki, Tsuyoshi

AU - Hirata, Takashi

PY - 2010/5

Y1 - 2010/5

N2 - We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants.

AB - We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants.

KW - Glycosphingolipids

KW - HPLC (High performance liquid chromatography)

KW - Mass spectrometry (MS)

KW - Plant lipids

KW - Sphingoid bases

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Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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