Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26

Keisuke Miyauchi; Masao Fukuda; Masataka Tsuda; Masamichi Takagi; Yuji Nagata

doi:10.1271/bbb.69.216

Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26

Keisuke Miyauchi, Masao Fukuda, Masataka Tsuda, Masamichi Takagi, Yuji Nagata

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, earned ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.

Original language	English
Pages (from-to)	216-219
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	69
Issue number	1
DOIs	https://doi.org/10.1271/bbb.69.216
Publication status	Published - 2005 Jan

Keywords

Biodegradation
Insertion sequence
Sphingomonas
Tn5 mutagenesis
γ-hexachlorocyclohexane

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26",

abstract = "Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, earned ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.",

keywords = "Biodegradation, Insertion sequence, Sphingomonas, Tn5 mutagenesis, γ-hexachlorocyclohexane",

author = "Keisuke Miyauchi and Masao Fukuda and Masataka Tsuda and Masamichi Takagi and Yuji Nagata",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. It was performed using the facilities of the Biotechnology Research Center of the University of Tokyo.",

year = "2005",

month = jan,

doi = "10.1271/bbb.69.216",

language = "English",

volume = "69",

pages = "216--219",

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T1 - Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26

AU - Miyauchi, Keisuke

AU - Fukuda, Masao

AU - Tsuda, Masataka

AU - Takagi, Masamichi

AU - Nagata, Yuji

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. It was performed using the facilities of the Biotechnology Research Center of the University of Tokyo.

PY - 2005/1

Y1 - 2005/1

N2 - Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, earned ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.

AB - Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, earned ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.

KW - Biodegradation

KW - Insertion sequence

KW - Sphingomonas

KW - Tn5 mutagenesis

KW - γ-hexachlorocyclohexane

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Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26

Abstract

Keywords

ASJC Scopus subject areas

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