TY - JOUR
T1 - Identification of six major outer membrane proteins from Actinobacillus actinomycetemcomitans
AU - Komatsuzawa, Hitoshi
AU - Asakawa, Ryuji
AU - Kawai, Toshihisa
AU - Ochiai, Kyoko
AU - Fujiwara, Tamaki
AU - Taubman, Martin A.
AU - Ohara, Masaru
AU - Kurihara, Hidemi
AU - Sugai, Motoyuki
N1 - Funding Information:
The research was supported in part by Grant 12357014 from the Ministry of Education, Science, Sports and Culture of Japan, and Grant DE-03420 from the National Institute of Dental and Craniofacial Research. Part of this study was carried out in the Research Center, the Research Facility for Laboratory Animal Science and the Research Facility, Hiroshima University Faculty of Dentistry.
PY - 2002/4/17
Y1 - 2002/4/17
N2 - We have identified six major sarcosyl-insoluble outer membrane proteins (Omp) of Actinobacillus actinomycetemcomitans Y4, and designated them as Omp100, Omp64, Omp39, Omp29, Omp18 and Omp16 according to the molecular mass. A similar N-terminal sequence was found in the first 15 amino acid residues of Omp16 and Omp18. The N-terminal sequence of Omp29 matched perfectly with the sequence previously identified. We cloned and determined the DNA sequences of three complete genes encoding Omp100, Omp64 and Omp18/16, and one incomplete gene encoding Omp39. Each Omp revealed homologies with some bacterial virulence factors responsible for adhesion, invasion, serum resistance, or protein antigenicity. Serum from patients with periodontitis suspected to be related to A. actinomycetemcomintans infection strongly reacted with Omp100, Omp29 and Omp16 as did serum from mice immunized with A. actinomycetemcomitans Y4 whole bacteria. These findings suggest that Omps of A. actinomycetemcomitans can be associated with periodontal disease.
AB - We have identified six major sarcosyl-insoluble outer membrane proteins (Omp) of Actinobacillus actinomycetemcomitans Y4, and designated them as Omp100, Omp64, Omp39, Omp29, Omp18 and Omp16 according to the molecular mass. A similar N-terminal sequence was found in the first 15 amino acid residues of Omp16 and Omp18. The N-terminal sequence of Omp29 matched perfectly with the sequence previously identified. We cloned and determined the DNA sequences of three complete genes encoding Omp100, Omp64 and Omp18/16, and one incomplete gene encoding Omp39. Each Omp revealed homologies with some bacterial virulence factors responsible for adhesion, invasion, serum resistance, or protein antigenicity. Serum from patients with periodontitis suspected to be related to A. actinomycetemcomintans infection strongly reacted with Omp100, Omp29 and Omp16 as did serum from mice immunized with A. actinomycetemcomitans Y4 whole bacteria. These findings suggest that Omps of A. actinomycetemcomitans can be associated with periodontal disease.
KW - Omp29
KW - Periodontitis
KW - Serum
KW - Virulence
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U2 - 10.1016/S0378-1119(02)00500-0
DO - 10.1016/S0378-1119(02)00500-0
M3 - Article
C2 - 12034509
AN - SCOPUS:0037123353
SN - 0378-1119
VL - 288
SP - 195
EP - 201
JO - Gene
JF - Gene
IS - 1-2
ER -