TY - JOUR
T1 - Identification of two novel members of erbA superfamily by molecular cloning
T2 - The gene products of the two are highly related to each other
AU - Miyajtma, Nobuyuki
AU - Kandowaki, Yasunori
AU - Fukushige, Shin Ichi
AU - Shimizu, Sho Ichi
AU - Semba, Kentaro
AU - Yamanashi, Yuji
AU - Matsubara, Ken Ichi
AU - Toyoshima, Kumao
AU - Yamamoto, Tadashi
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank Y. Yamamuro for her help in preparation of the manuscript. This work was supported in part by a Grant-in-Aid for Special Project Research Cancer-Bioscience from the Ministry of Education, Science, and Culture of Japan.
PY - 1988/12/9
Y1 - 1988/12/9
N2 - Two v-erbA-related genes, named ear-2 and ear-3, have been identified in the human genome and characterized by cDNA cloning. These genes are predicted to encode proteins that are very similar in primary structure to receptors for steroid hormones or thyroid hormone (T3) In addition, amino acid sequences of the ear-2 and ear-3 gene products are very similar each other especially at the DNA binding domain (86% homology) and at the putative ligand binding domain (76% homology). Northern hybridization with ear DNA probes of RNAs from various tissues of a human fetus reveals that the expression of ear-2 is high in the liver whereas the expression of ear-3 is relatively ubiquitous. Hybridization analysis of DNAs from sorted chromosomes shows that the ear-2 gene is located on chromosome 19 and ear-3 on chromosome 5, indicating that the two genes are clearly different from each other.
AB - Two v-erbA-related genes, named ear-2 and ear-3, have been identified in the human genome and characterized by cDNA cloning. These genes are predicted to encode proteins that are very similar in primary structure to receptors for steroid hormones or thyroid hormone (T3) In addition, amino acid sequences of the ear-2 and ear-3 gene products are very similar each other especially at the DNA binding domain (86% homology) and at the putative ligand binding domain (76% homology). Northern hybridization with ear DNA probes of RNAs from various tissues of a human fetus reveals that the expression of ear-2 is high in the liver whereas the expression of ear-3 is relatively ubiquitous. Hybridization analysis of DNAs from sorted chromosomes shows that the ear-2 gene is located on chromosome 19 and ear-3 on chromosome 5, indicating that the two genes are clearly different from each other.
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U2 - 10.1093/nar/16.23.11057
DO - 10.1093/nar/16.23.11057
M3 - Article
C2 - 2905047
AN - SCOPUS:0024241354
SN - 0305-1048
VL - 16
SP - 11057
EP - 11074
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 23
ER -