TY - JOUR
T1 - Identification of two α-subunit species of GTP-binding proteins, Gα15 and Gαq, expressed in rat taste buds
AU - Kusakabe, Yuko
AU - Yamaguchi, Eiri
AU - Tanemura, Kentaro
AU - Kameyama, Kimihiko
AU - Chiba, Noboru
AU - Arai, Soichi
AU - Emori, Yasufumi
AU - Abe, Keiko
N1 - Funding Information:
This study was supported in part by a grant from the Program for the Promotion of Basic Research Activities for Innovation Biosciences.
PY - 1998/7/24
Y1 - 1998/7/24
N2 - We cloned cDNAs for two G protein α-subunits belonging to the Gαq family, each capable of activating PLCβ, from rat tongue. One is a Gαq in the narrow sense, and the other, termed rat Gα15, is a rat counterpart of mouse Gα15, sharing an amino acid sequence similarity of 94%. RT-PCR and Northern blot analysis demonstrated that rat Gα15 and Gαq were distinctly expressed in tongue epithelia containing taste buds. Immunostaining also showed that rat Gα15, together with the Gαq, was localized mainly in taste buds. These studies suggest the possibility that these two Gα proteins function for taste signal transduction in sensory cells. Copyright (C) 1998 Elsevier Science B.V.
AB - We cloned cDNAs for two G protein α-subunits belonging to the Gαq family, each capable of activating PLCβ, from rat tongue. One is a Gαq in the narrow sense, and the other, termed rat Gα15, is a rat counterpart of mouse Gα15, sharing an amino acid sequence similarity of 94%. RT-PCR and Northern blot analysis demonstrated that rat Gα15 and Gαq were distinctly expressed in tongue epithelia containing taste buds. Immunostaining also showed that rat Gα15, together with the Gαq, was localized mainly in taste buds. These studies suggest the possibility that these two Gα proteins function for taste signal transduction in sensory cells. Copyright (C) 1998 Elsevier Science B.V.
KW - G protein
KW - Gαq family
KW - PLCβ
KW - Taste bud
KW - Taste signal transduction
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U2 - 10.1016/S0167-4889(98)00062-7
DO - 10.1016/S0167-4889(98)00062-7
M3 - Article
C2 - 9685675
AN - SCOPUS:0344936733
SN - 0167-4889
VL - 1403
SP - 265
EP - 272
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 3
ER -