@article{a2bf658663674edd9cdde3a1a79dd23e,
title = "Illuminating G-Protein-Coupling Selectivity of GPCRs",
abstract = "A large-scale functional interaction study reveals how GPCRs engage with subsets of G(alpha) proteins and outlines characteristic predictive of these associations.",
keywords = "DREADD, G-protein-coupled receptors, HEK293 cells, NanoBiT, TGF-α shedding assay, bioinformatics, chimeric G protein, prediction, protein design, signaling",
author = "Asuka Inoue and Francesco Raimondi and Kadji, {Francois Marie Ngako} and Gurdeep Singh and Takayuki Kishi and Akiharu Uwamizu and Yuki Ono and Yuji Shinjo and Satoru Ishida and Nadia Arang and Kouki Kawakami and Gutkind, {J. Silvio} and Junken Aoki and Russell, {Robert B.}",
note = "Funding Information: We thank Frederick J. Ehlert at University of California, Irvine and Sudarshan Rajagopal at Duke University for helpful comments on analyses of the chimeric G-protein-based assay; Michel Bouvier at University of Montreal for discussion on G-protein profiling; members of the laboratory at Tohoku University for plasmid preparation; Ayumi Inoue at Tohoku University for flow cytometry analyses. This work was supported by JSPS KAKENHI grant 17K08264 (A.I.); Japan Science and Technology Agency (JST), PRESTO JPMJPR1331 (A.I.); and the PRIME JP17gm5910013 (A.I.) and the LEAP JP17gm0010004 (A.I. and J.A.) from the Japan Agency for Medical Research and Development (AMED). N.A. is supported by training grants from the National Institute of General Medical Sciences (NIGMS) T32GM007752 and the National Science Foundation (NSF) DGE-1650112 . J.S.G. is supported by relevant grants from the National Cancer Institute (NCI) R33CA225291 and U54CA209891 ; and the National Institute of Dental and Craniofacial Research (NIDCR) U01DE028227 . F.R., G.S., and R.B.R. are supported by the Cell Networks Excellence initiative of the Germany Research Foundation (DFG). R.B.R. is part of the DFG SFB/TPR186 Molecular Switches in the Spatio-Temporal Control of Cellular Signal Transmission and the BMBF German Network for Bioinformatics (de.NBI). F.R. was additionally supported by an Alexander Von Humboldt post-doctoral fellowship (F.R.) and a Michael J. Fox Foundation research grant (F.R. and R.B.R.). Funding Information: We thank Frederick J. Ehlert at University of California, Irvine and Sudarshan Rajagopal at Duke University for helpful comments on analyses of the chimeric G-protein-based assay; Michel Bouvier at University of Montreal for discussion on G-protein profiling; members of the laboratory at Tohoku University for plasmid preparation; Ayumi Inoue at Tohoku University for flow cytometry analyses. This work was supported by JSPS KAKENHI grant 17K08264 (A.I.); Japan Science and Technology Agency (JST), PRESTO JPMJPR1331 (A.I.); and the PRIME JP17gm5910013 (A.I.) and the LEAP JP17gm0010004 (A.I. and J.A.) from the Japan Agency for Medical Research and Development (AMED). N.A. is supported by training grants from the National Institute of General Medical Sciences (NIGMS) T32GM007752 and the National Science Foundation (NSF) DGE-1650112. J.S.G. is supported by relevant grants from the National Cancer Institute (NCI) R33CA225291 and U54CA209891; and the National Institute of Dental and Craniofacial Research (NIDCR) U01DE028227. F.R. G.S. and R.B.R. are supported by the Cell Networks Excellence initiative of the Germany Research Foundation (DFG). R.B.R. is part of the DFG SFB/TPR186 Molecular Switches in the Spatio-Temporal Control of Cellular Signal Transmission and the BMBF German Network for Bioinformatics (de.NBI). F.R. was additionally supported by an Alexander Von Humboldt post-doctoral fellowship (F.R.) and a Michael J. Fox Foundation research grant (F.R. and R.B.R.). Conceptualization, A.I. and F.R.; Methodology, A.I. F.R. G.S. J.A. and R.B.R; Investigation, A.I. F.M.N.K. Y.S. S.I. T.K. F.R. A.U. Y.O. N.A. K.K. G.S. and J.S.G.; Writing, A.I. F.R. and R.B.R. with feedback from all of the coauthors; Funding Acquisition, A.I. J.A. F.R. and R.B.R.; Resources, A.I.; Supervision, A.I. F.R. and R.B.R. A.I. F.R. J.A. and R.B.R. have filed a patent application related to this work. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jun,
day = "13",
doi = "10.1016/j.cell.2019.04.044",
language = "English",
volume = "177",
pages = "1933--1947.e25",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "7",
}