TY - JOUR
T1 - Imaging of the cortical cytoskeleton of guinea pig outer hair cells using atomic force microscopy
AU - Wada, Hiroshi
AU - Kimura, Kei
AU - Gomi, Takashi
AU - Sugawara, Michiko
AU - Katori, Yukio
AU - Kakehata, Seiji
AU - Ikeda, Katsuhisa
AU - Kobayashi, Toshimitsu
N1 - Funding Information:
This work was supported by a grant from the Human Frontier Science Program, by a Health and Labour Science Research Grant from the Ministry of Health, Labour and Welfare of Japan, by a Grant-in-Aid for Scientific Research (A) 11307033, and a Grant-in-Aid for Scientific Research on Priority Areas 15086202 from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2004/1
Y1 - 2004/1
N2 - Mammalian outer hair cells (OHCs) are known to respond to acoustical stimulation with elongation and contraction of the cells' cylindrical soma in vivo, and this motility is related to both the protein motors distributed along the OHC plasma membrane and the cytoskeleton beneath it. Therefore, the cytoskeleton seems to play an important role in the motility of the OHC. Recently, an atomic force microscope (AFM) was used to investigate the OHC cytoskeleton under physiological conditions. However, details were not made clear in that study. In this study, the ultrastructure of the cytoskeleton of fixed OHCs of guinea pigs, which were extracted with Triton X-100, was investigated using the AFM. As a result, the cortical cytoskeleton, which is formed by discrete oriented domains, was imaged, and circumferential filaments and cross-links were observed within the domain. Morphological change of the cytoskeleton of the OHC induced by diamide treatment was then examined using the AFM, and reduction of cross-links was observed. The examination indicates that the cortical cytoskeleton comprises circumferential actin filaments and spectrin cross-links.
AB - Mammalian outer hair cells (OHCs) are known to respond to acoustical stimulation with elongation and contraction of the cells' cylindrical soma in vivo, and this motility is related to both the protein motors distributed along the OHC plasma membrane and the cytoskeleton beneath it. Therefore, the cytoskeleton seems to play an important role in the motility of the OHC. Recently, an atomic force microscope (AFM) was used to investigate the OHC cytoskeleton under physiological conditions. However, details were not made clear in that study. In this study, the ultrastructure of the cytoskeleton of fixed OHCs of guinea pigs, which were extracted with Triton X-100, was investigated using the AFM. As a result, the cortical cytoskeleton, which is formed by discrete oriented domains, was imaged, and circumferential filaments and cross-links were observed within the domain. Morphological change of the cytoskeleton of the OHC induced by diamide treatment was then examined using the AFM, and reduction of cross-links was observed. The examination indicates that the cortical cytoskeleton comprises circumferential actin filaments and spectrin cross-links.
KW - Actin filament
KW - Atomic force microscope
KW - Cortical lattice
KW - Outer hair cell
KW - Spectrin cross-link
UR - http://www.scopus.com/inward/record.url?scp=0345862386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345862386&partnerID=8YFLogxK
U2 - 10.1016/S0378-5955(03)00334-4
DO - 10.1016/S0378-5955(03)00334-4
M3 - Article
AN - SCOPUS:0345862386
SN - 0378-5955
VL - 187
SP - 51
EP - 62
JO - Hearing Research
JF - Hearing Research
IS - 1-2
ER -