Immobilization of proteins on solid substrate employing affinity tags for surface forces measurement

Yoshiki Nakata; Takahiro Yamaguchi; Yuichiro Shimzu; Takehiro Suzuki; Kazuya Morikawa; Masaya Fujita; Kazue Kurihara

Immobilization of proteins on solid substrate employing affinity tags for surface forces measurement

Yoshiki Nakata, Takahiro Yamaguchi, Yuichiro Shimzu, Takehiro Suzuki, Kazuya Morikawa, Masaya Fujita, Kazue Kurihara

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to conference › Paper › peer-review

Abstract

Proteins play significant roles in living systems through their interactions. So it is important to understand the interactions between them. For studying protein interactions by the surface forces measurement, it is essential to immobilize them on substrates. We immobilized proteins on the substrates employing affinity tags and measured protein interactions using colloid probe atomic force microscopy. This time, the interactions of SigB/RsbW and Spo0A-DB/DNA were studied.

Original language	English
Pages	5434-5435
Number of pages	2
Publication status	Published - 2006
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 2006 Sept 20 → 2006 Sept 22

Conference

Conference	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	06/9/20 → 06/9/22

Keywords

Affinity tag
Atomic force microscopy
Immobilization
Protein
Surface force

Cite this

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title = "Immobilization of proteins on solid substrate employing affinity tags for surface forces measurement",

abstract = "Proteins play significant roles in living systems through their interactions. So it is important to understand the interactions between them. For studying protein interactions by the surface forces measurement, it is essential to immobilize them on substrates. We immobilized proteins on the substrates employing affinity tags and measured protein interactions using colloid probe atomic force microscopy. This time, the interactions of SigB/RsbW and Spo0A-DB/DNA were studied.",

keywords = "Affinity tag, Atomic force microscopy, Immobilization, Protein, Surface force",

author = "Yoshiki Nakata and Takahiro Yamaguchi and Yuichiro Shimzu and Takehiro Suzuki and Kazuya Morikawa and Masaya Fujita and Kazue Kurihara",

year = "2006",

language = "English",

pages = "5434--5435",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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TY - CONF

T1 - Immobilization of proteins on solid substrate employing affinity tags for surface forces measurement

AU - Nakata, Yoshiki

AU - Yamaguchi, Takahiro

AU - Shimzu, Yuichiro

AU - Suzuki, Takehiro

AU - Morikawa, Kazuya

AU - Fujita, Masaya

AU - Kurihara, Kazue

PY - 2006

Y1 - 2006

N2 - Proteins play significant roles in living systems through their interactions. So it is important to understand the interactions between them. For studying protein interactions by the surface forces measurement, it is essential to immobilize them on substrates. We immobilized proteins on the substrates employing affinity tags and measured protein interactions using colloid probe atomic force microscopy. This time, the interactions of SigB/RsbW and Spo0A-DB/DNA were studied.

AB - Proteins play significant roles in living systems through their interactions. So it is important to understand the interactions between them. For studying protein interactions by the surface forces measurement, it is essential to immobilize them on substrates. We immobilized proteins on the substrates employing affinity tags and measured protein interactions using colloid probe atomic force microscopy. This time, the interactions of SigB/RsbW and Spo0A-DB/DNA were studied.

KW - Affinity tag

KW - Atomic force microscopy

KW - Immobilization

KW - Protein

KW - Surface force

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UR - http://www.scopus.com/inward/citedby.url?scp=33846144216&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33846144216

SP - 5434

EP - 5435

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Immobilization of proteins on solid substrate employing affinity tags for surface forces measurement

Abstract

Conference

Keywords

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