Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors

Ioulia G. Mourzina; Tatsuo Yoshinobu; Yuri E. Ermolenko; Yuri G. Vlasov; Michael J. Schöning; Hiroshi Iwasaki

doi:10.1007/s00604-003-0091-4

Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors

Ioulia G. Mourzina, Tatsuo Yoshinobu, Yuri E. Ermolenko, Yuri G. Vlasov, Michael J. Schöning, Hiroshi Iwasaki

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Various methods for the immobilization of urease and butyrylcholinesterase on the insulator surface of a laser-scanned semiconductor transducer (LSST) have been tested and compared for the development of an enzyme-based light-addressable potentiometric sensor (LAPS). The method of preparing photocurable membranes on LAPS is presented, and a new type of enzyme LAPS with photocurable polymeric enzyme membranes has been elaborated. It was found that sensors prepared by means of covalent bonding and cross-linking with inactive protein (type SIII) and with photocurable membrane matrices (type SIV) are more prospective. The enzyme LAPSensors with photocurable membranes demonstrate a degree of sensitivity close to the theoretical value and working ranges of 6.3•10^-5-1.1•10^-2 and 1•10 ^-4-1•10^-1∈mol∈L^-1 urea for acrylamide and acrylate-based membrane matrices, respectively, and 2.5•10^-4-2•10^-1∈mol∈L^-1 butyrylcholine for an acrylamide membrane matrix. It is shown that such sensors can be also used for the analysis of enzyme inhibitors.

Original language	English
Pages (from-to)	41-50
Number of pages	10
Journal	Microchimica Acta
Volume	144
Issue number	1-3
DOIs	https://doi.org/10.1007/s00604-003-0091-4
Publication status	Published - 2004
Externally published	Yes

Keywords

Enzyme immobilisation
Enzyme inhibitor
Enzyme-LAPS
Light-addressable potentiometric sensor
Photocurable polymer

ASJC Scopus subject areas

Analytical Chemistry

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10.1007/s00604-003-0091-4

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title = "Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors",

abstract = "Various methods for the immobilization of urease and butyrylcholinesterase on the insulator surface of a laser-scanned semiconductor transducer (LSST) have been tested and compared for the development of an enzyme-based light-addressable potentiometric sensor (LAPS). The method of preparing photocurable membranes on LAPS is presented, and a new type of enzyme LAPS with photocurable polymeric enzyme membranes has been elaborated. It was found that sensors prepared by means of covalent bonding and cross-linking with inactive protein (type SIII) and with photocurable membrane matrices (type SIV) are more prospective. The enzyme LAPSensors with photocurable membranes demonstrate a degree of sensitivity close to the theoretical value and working ranges of 6.3•10-5-1.1•10-2 and 1•10 -4-1•10-1∈mol∈L-1 urea for acrylamide and acrylate-based membrane matrices, respectively, and 2.5•10-4-2•10-1∈mol∈L-1 butyrylcholine for an acrylamide membrane matrix. It is shown that such sensors can be also used for the analysis of enzyme inhibitors.",

keywords = "Enzyme immobilisation, Enzyme inhibitor, Enzyme-LAPS, Light-addressable potentiometric sensor, Photocurable polymer",

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AU - Mourzina, Ioulia G.

AU - Yoshinobu, Tatsuo

AU - Ermolenko, Yuri E.

AU - Vlasov, Yuri G.

AU - Schöning, Michael J.

AU - Iwasaki, Hiroshi

PY - 2004

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Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors

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