@inbook{1fb3f72e0c7f434eb99f6d6e5d141266,
title = "Immuno-northern blotting: Detection of modified RNA using gel separation and antibodies to modified nucleosides",
abstract = "Immuno-northern blotting is a method for detecting modified RNAs using gel separation and specific antibodies to modified nucleosides. This method was developed by combining two commonly used molecular biology techniques: western blotting and northern blotting. In this method, urea-polyacrylamide (or agarose) gel-separated RNAs are transferred to positively charged nylon membrane and then immune detection is performed with specific antibodies to modified nucleosides: such as 1-methyladenosine, N6-methyladenosine, and pseudouridine. This highly sensitive and relatively simple method, which uses widely available laboratory equipment, enables small laboratories to compare the abundance of modified nucleic acids across samples.",
keywords = "1-Methyladenosine, Modified nucleotide, N6-Methyladenosine, Pseudouridine, RNA modification, Small RNAs, tRNA",
author = "Eikan Mishima and Takaaki Abe",
note = "Funding Information: This work was supported in part by the Japan Society for the Promotion of Science (JSPS) KAKENHI (26860624 to E.M.). Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-8808-2_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "179--187",
booktitle = "Methods in Molecular Biology",
}