TY - JOUR
T1 - Immunocytochemical Analysis of DGKη in Cultured Cells Using a Monoclonal Antibody DhMab-4
AU - Nakano, Tomoyuki
AU - Tanaka, Toshiaki
AU - Sakane, Fumio
AU - Kaneko, Mika K.
AU - Kato, Yukinari
AU - Goto, Kaoru
N1 - Funding Information:
This study was supported in part by AMED under Grant No. JP21am0101078 (Y.K.) and by the grant-in-aid from MEXT (T.N. and K.G.).
Funding Information:
K.G. and Y.K. received research funding from Ono Pharmaceutical Co., Ltd. The other authors have no conflict of interest.
Publisher Copyright:
© Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.
PY - 2021/12
Y1 - 2021/12
N2 - Diacylglycerol kinase (DGK) is a lipid kinase that converts diacylglycerol (DG) to phosphatidic acid (PA). Since both DG and PA serve as intracellular second messenger molecules, DGK plays a pivotal role in balancing these two signaling pathways. Of the DGK family, DGKη is classified as a type II DGK. Reportedly, DGKη is expressed ubiquitously through mammalian tissues and cells. Previous studies using cDNA transfection methods reported cytoplasmic localization of DGKη in cultured human cells. However, subcellular localization of native protein is still unknown. Recently, we established a human DGKη-specific monoclonal antibody, DhMab-4. In this study, we examined subcellular localization of native protein of DGKη using DhMab-4 by immunocytochemistry in human cultured cells.
AB - Diacylglycerol kinase (DGK) is a lipid kinase that converts diacylglycerol (DG) to phosphatidic acid (PA). Since both DG and PA serve as intracellular second messenger molecules, DGK plays a pivotal role in balancing these two signaling pathways. Of the DGK family, DGKη is classified as a type II DGK. Reportedly, DGKη is expressed ubiquitously through mammalian tissues and cells. Previous studies using cDNA transfection methods reported cytoplasmic localization of DGKη in cultured human cells. However, subcellular localization of native protein is still unknown. Recently, we established a human DGKη-specific monoclonal antibody, DhMab-4. In this study, we examined subcellular localization of native protein of DGKη using DhMab-4 by immunocytochemistry in human cultured cells.
KW - DGKη
KW - immunocytochemical analysis
KW - monoclonal antibody
UR - http://www.scopus.com/inward/record.url?scp=85122378039&partnerID=8YFLogxK
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U2 - 10.1089/mab.2021.0034
DO - 10.1089/mab.2021.0034
M3 - Article
C2 - 34958278
AN - SCOPUS:85122378039
SN - 2167-9436
VL - 40
SP - 261
EP - 265
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 6
ER -