Immunocytochemical Analysis of DGKη in Cultured Cells Using a Monoclonal Antibody DhMab-4

Tomoyuki Nakano, Toshiaki Tanaka, Fumio Sakane, Mika K. Kaneko, Yukinari Kato, Kaoru Goto

Research output: Contribution to journalArticlepeer-review


Diacylglycerol kinase (DGK) is a lipid kinase that converts diacylglycerol (DG) to phosphatidic acid (PA). Since both DG and PA serve as intracellular second messenger molecules, DGK plays a pivotal role in balancing these two signaling pathways. Of the DGK family, DGKη is classified as a type II DGK. Reportedly, DGKη is expressed ubiquitously through mammalian tissues and cells. Previous studies using cDNA transfection methods reported cytoplasmic localization of DGKη in cultured human cells. However, subcellular localization of native protein is still unknown. Recently, we established a human DGKη-specific monoclonal antibody, DhMab-4. In this study, we examined subcellular localization of native protein of DGKη using DhMab-4 by immunocytochemistry in human cultured cells.

Original languageEnglish
Pages (from-to)261-265
Number of pages5
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Issue number6
Publication statusPublished - 2021 Dec


  • DGKη
  • immunocytochemical analysis
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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