TY - JOUR
T1 - Immunohistochemical Detection of Sheep Podoplanin Using an Antibovine Podoplanin Monoclonal Antibody PMab-44
AU - Kato, Yukinari
AU - Yamada, Shinji
AU - Itai, Shunsuke
AU - Kobayashi, Atsushi
AU - Konnai, Satoru
AU - Kaneko, Mika K.
N1 - Funding Information:
This research was supported, in part, by AMED under grant numbers: JP18am0101078 (Y.K.), JP18am0301010 (Y.K.), and JP18ae0101028 (Y.K.), and by JSPS KAKENHI grant number 17K07299 (M.K.K.) and grant number 16K10748 (Y.K.).
Publisher Copyright:
© Copyright 2018, Mary Ann Liebert, Inc., publishers 2018.
PY - 2018/12
Y1 - 2018/12
N2 - Podoplanin (PDPN) obtained from various animal species has been characterized using specific anti-PDPN monoclonal antibodies (mAbs), namely, PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 against mouse, rat, rabbit, dog, bovine, and cat PDPN, respectively. PDPN is expressed in type I alveolar cells in lungs, lymphatic endothelial cells, and kidney podocytes. In this study, we investigated possible cross-reactions between anti-PDPN mAbs and sheep PDPN. Type I alveolar cells from sheep lung were strongly detected by PMab-44 using immunohistochemical analyses. These results indicate that PMab-44 may be useful for the detection of sheep PDPN.
AB - Podoplanin (PDPN) obtained from various animal species has been characterized using specific anti-PDPN monoclonal antibodies (mAbs), namely, PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 against mouse, rat, rabbit, dog, bovine, and cat PDPN, respectively. PDPN is expressed in type I alveolar cells in lungs, lymphatic endothelial cells, and kidney podocytes. In this study, we investigated possible cross-reactions between anti-PDPN mAbs and sheep PDPN. Type I alveolar cells from sheep lung were strongly detected by PMab-44 using immunohistochemical analyses. These results indicate that PMab-44 may be useful for the detection of sheep PDPN.
KW - PMab-44
KW - sheep PDPN
KW - sheep podoplanin
UR - http://www.scopus.com/inward/record.url?scp=85059232507&partnerID=8YFLogxK
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U2 - 10.1089/mab.2018.0036
DO - 10.1089/mab.2018.0036
M3 - Article
C2 - 30570359
AN - SCOPUS:85059232507
SN - 2167-9436
VL - 37
SP - 265
EP - 268
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 6
ER -