TY - JOUR
T1 - Immunohistochemical distribution and functional characterization of an organic anion transporting polypeptide 2 (oatp2)
AU - Kakyo, Masayuki
AU - Sakagami, Hiroyuki
AU - Nishio, Toshiyuki
AU - Nakai, Daisuke
AU - Nakagomi, Rie
AU - Tokui, Taro
AU - Naitoh, Takeshi
AU - Matsuno, Seiki
AU - Abe, Takaaki
AU - Yawo, Hiromu
N1 - Funding Information:
We thank Dr. Kazuo Nunoki for critical reading of the manuscript. This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Yamanouchi Foundation for Research on Metabolic Disorders, the Nishimiya Foundation, the Japan Research Foundation for Clinical Pharmacology and the Inamori Foundation.
PY - 1999/2/26
Y1 - 1999/2/26
N2 - The rabbit polyclonal antibody against rat organic anion transporting polypeptide 2 (oatp2) was raised and immunoaffinity-purified. Western blot analysis for oatp2 detected two bands (~74 and 76 kDa) in rat brain and a single band (76 kDa) in the liver. By immunohistochemical analysis, the oatp2 immunoreactivity was specifically high at the basolateral membrane of rat hepatocytes. Functionally, the oatp2-expressing oocytes were found to transport dehydroepiandrosterone sulfate, δ1 opioid receptor agonist [d-Pen2,d-Pen5]enkephalin, Leu-enkephalin, and biotin significantly, as well as the substrates previously reported. These data reveal the exact distribution of the rat oatp2 at the protein level in the liver, and that oatp2 appears to be involved in the multispecificity of the uptaking substrates in the liver and brain. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - The rabbit polyclonal antibody against rat organic anion transporting polypeptide 2 (oatp2) was raised and immunoaffinity-purified. Western blot analysis for oatp2 detected two bands (~74 and 76 kDa) in rat brain and a single band (76 kDa) in the liver. By immunohistochemical analysis, the oatp2 immunoreactivity was specifically high at the basolateral membrane of rat hepatocytes. Functionally, the oatp2-expressing oocytes were found to transport dehydroepiandrosterone sulfate, δ1 opioid receptor agonist [d-Pen2,d-Pen5]enkephalin, Leu-enkephalin, and biotin significantly, as well as the substrates previously reported. These data reveal the exact distribution of the rat oatp2 at the protein level in the liver, and that oatp2 appears to be involved in the multispecificity of the uptaking substrates in the liver and brain. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - [d-Pen,d-Pen]Enkephalin
KW - Basolateral membrane
KW - Dehydroepiandrosterone sulfate
KW - Organic anion transporting polypeptide 2
KW - Rat liver
KW - Steroid hormone
KW - Thyroid hormone
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U2 - 10.1016/S0014-5793(99)00152-0
DO - 10.1016/S0014-5793(99)00152-0
M3 - Article
C2 - 10094486
AN - SCOPUS:0033054241
SN - 0014-5793
VL - 445
SP - 343
EP - 346
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -