Immunohistochemical study of 3β-hydroxysteroid dehydrogenase in sex cord-stromal tumors of the ovary

Hironobu Sasano, J. Ian Mason, Etsuko Sasaki, Akira Yajima, Noriko Kimura, Tsuneo Namiki, Nobuaki Sasano, Hiroshi Nagura

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4 Citations (Scopus)


3β-Hydroxysteroid dehydrogenase (3β-HSD), which converts pregnenolone to progesterone, was localized immunohistochemically in 18 thecomas, 23 fibromas, 5 granulosa-cell tumors, 5 sclerosing stromal tumors, and 2 steroid-cell tumors. Immunohistochemical study of estrogen, progesterone, and testosterone was also performed in serial sections of thecomas and fibromas. In thecomas, immunoreactivity of 3β-HSD was observed only in luteinized theca cells and thecomatous tumor cells with abundant pale to vacuolated cytoplasm but not in spindled tumor cells and thecomatous tumor cells with small to moderate amounts of pale to vacuolated cytoplasm. Immunoreactivity of steroids was not observed in thecomas except for testosterone immunoreactivity in one case. No immunoreactivity of steroids or the enzyme was present in fibromas. No tumor cells were positive for 3β-HSD in any of the cases of granulosa-cell tumor examined. Immunoreactivity of 3β-HSD was present in cells in steroid-cell tumors and polygonal tumor cells with prominent cytoplasmic vacuoles in two cases of sclerosing stromal tumor. Thus, 3β-HSD can be a good immunohistochemical marker of steroidogenesis in functioning ovarian neoplasms.

Original languageEnglish
Pages (from-to)352-362
Number of pages11
JournalInternational Journal of Gynecological Pathology
Issue number4
Publication statusPublished - 1990 Oct


  • 3β-Hydroxysteroid dehydrogenase
  • Immunohistochemistry
  • Ovarian neoplasms
  • Steroidogenesis
  • Thecoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynaecology


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