Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells

Toyoaki Anai; Evalour T. Aspuria; Nobuharu Fujii; Takashi Ueda; Minami Matsui; Koji Hasegawa; Hirofumi Uchimiya

doi:10.1016/S0176-1617(11)81411-2

Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells

Toyoaki Anai, Evalour T. Aspuria, Nobuharu Fujii, Takashi Ueda, Minami Matsui, Koji Hasegawa, Hirofumi Uchimiya

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

ara genes encoding YPT/RAB like small GTP-binding proteins, which are involved in the vesicular transport system in various eukaryotic cells, have been isolated from Arabidopsis thaliana. To determine the subcellular localization of ARA-4 protein, suspensions of cultured transgenic tobacco cells were analyzed using monoclonal antibody to Arabidopsis thaliana ARA-4 protein. ARA-4 protein was found in the microsomal membrane fraction. Similar results were obtained withArabidopsis thaliana cells. The ARA-4 protein was readily released by detergents, such as 0.5 % sodium deoxycholate and NP-40. The results of immuno-blot analysis with extracts of different organs show that the ARA-4 protein is present in most organs of Arabidopsis thaliana plants, e. g. root, stem, flower and young fruit, at the same level in all organs except for root, where the level is lower.

Original language	English
Pages (from-to)	48-52
Number of pages	5
Journal	Journal of Plant Physiology
Volume	147
Issue number	1
DOIs	https://doi.org/10.1016/S0176-1617(11)81411-2
Publication status	Published - 1995
Externally published	Yes

Keywords

5-bromo-4-chloro-3-indolyl phosphate
A rabidopsis thaliana
BCIP
CBB
GST
IPTG
NBT
Nicotiana tabacum
PVDF
SDS-PAGE
YPT/RAB
coomassie brilliant blue
glutathione S-transferase
isopropyl-β-D-thiogalactopyranoside
monoclonal antibody
nitro blue tetrazolium
polyvinylidene difluoride
small GTP-binding protein
sodium dodecyl sulfate polyacrylamide gel electrophoresis
transgenic plant

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/S0176-1617(11)81411-2

Cite this

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title = "Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells",

abstract = "ara genes encoding YPT/RAB like small GTP-binding proteins, which are involved in the vesicular transport system in various eukaryotic cells, have been isolated from Arabidopsis thaliana. To determine the subcellular localization of ARA-4 protein, suspensions of cultured transgenic tobacco cells were analyzed using monoclonal antibody to Arabidopsis thaliana ARA-4 protein. ARA-4 protein was found in the microsomal membrane fraction. Similar results were obtained withArabidopsis thaliana cells. The ARA-4 protein was readily released by detergents, such as 0.5 % sodium deoxycholate and NP-40. The results of immuno-blot analysis with extracts of different organs show that the ARA-4 protein is present in most organs of Arabidopsis thaliana plants, e. g. root, stem, flower and young fruit, at the same level in all organs except for root, where the level is lower.",

keywords = "5-bromo-4-chloro-3-indolyl phosphate, A rabidopsis thaliana, BCIP, CBB, GST, IPTG, NBT, Nicotiana tabacum, PVDF, SDS-PAGE, YPT/RAB, coomassie brilliant blue, glutathione S-transferase, isopropyl-β-D-thiogalactopyranoside, monoclonal antibody, nitro blue tetrazolium, polyvinylidene difluoride, small GTP-binding protein, sodium dodecyl sulfate polyacrylamide gel electrophoresis, transgenic plant",

author = "Toyoaki Anai and Aspuria, {Evalour T.} and Nobuharu Fujii and Takashi Ueda and Minami Matsui and Koji Hasegawa and Hirofumi Uchimiya",

note = "Funding Information: This research was jointly supported by a Grants-Aid for Scientific Research, Ministry of Education, Culture and Science, Japan, and a grant of Toray Science Foundation.",

year = "1995",

doi = "10.1016/S0176-1617(11)81411-2",

language = "English",

volume = "147",

pages = "48--52",

journal = "Z. PFLANZENPHYSIOL.",

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number = "1",

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TY - JOUR

T1 - Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells

AU - Anai, Toyoaki

AU - Aspuria, Evalour T.

AU - Fujii, Nobuharu

AU - Ueda, Takashi

AU - Matsui, Minami

AU - Hasegawa, Koji

AU - Uchimiya, Hirofumi

N1 - Funding Information: This research was jointly supported by a Grants-Aid for Scientific Research, Ministry of Education, Culture and Science, Japan, and a grant of Toray Science Foundation.

PY - 1995

Y1 - 1995

N2 - ara genes encoding YPT/RAB like small GTP-binding proteins, which are involved in the vesicular transport system in various eukaryotic cells, have been isolated from Arabidopsis thaliana. To determine the subcellular localization of ARA-4 protein, suspensions of cultured transgenic tobacco cells were analyzed using monoclonal antibody to Arabidopsis thaliana ARA-4 protein. ARA-4 protein was found in the microsomal membrane fraction. Similar results were obtained withArabidopsis thaliana cells. The ARA-4 protein was readily released by detergents, such as 0.5 % sodium deoxycholate and NP-40. The results of immuno-blot analysis with extracts of different organs show that the ARA-4 protein is present in most organs of Arabidopsis thaliana plants, e. g. root, stem, flower and young fruit, at the same level in all organs except for root, where the level is lower.

AB - ara genes encoding YPT/RAB like small GTP-binding proteins, which are involved in the vesicular transport system in various eukaryotic cells, have been isolated from Arabidopsis thaliana. To determine the subcellular localization of ARA-4 protein, suspensions of cultured transgenic tobacco cells were analyzed using monoclonal antibody to Arabidopsis thaliana ARA-4 protein. ARA-4 protein was found in the microsomal membrane fraction. Similar results were obtained withArabidopsis thaliana cells. The ARA-4 protein was readily released by detergents, such as 0.5 % sodium deoxycholate and NP-40. The results of immuno-blot analysis with extracts of different organs show that the ARA-4 protein is present in most organs of Arabidopsis thaliana plants, e. g. root, stem, flower and young fruit, at the same level in all organs except for root, where the level is lower.

KW - 5-bromo-4-chloro-3-indolyl phosphate

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KW - BCIP

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KW - GST

KW - IPTG

KW - NBT

KW - Nicotiana tabacum

KW - PVDF

KW - SDS-PAGE

KW - YPT/RAB

KW - coomassie brilliant blue

KW - glutathione S-transferase

KW - isopropyl-β-D-thiogalactopyranoside

KW - monoclonal antibody

KW - nitro blue tetrazolium

KW - polyvinylidene difluoride

KW - small GTP-binding protein

KW - sodium dodecyl sulfate polyacrylamide gel electrophoresis

KW - transgenic plant

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Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells

Abstract

Keywords

ASJC Scopus subject areas

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