Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees

Miyoshi Haruta; Masatsune Murata; Hiroshi Kadokura; Seiichi Homma

doi:10.1016/S0031-9422(98)00633-5

Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees

Miyoshi Haruta, Masatsune Murata, Hiroshi Kadokura, Seiichi Homma

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.

Original language	English
Pages (from-to)	1021-1025
Number of pages	5
Journal	Phytochemistry
Volume	50
Issue number	6
DOIs	https://doi.org/10.1016/S0031-9422(98)00633-5
Publication status	Published - 1999 Mar 20
Externally published	Yes

Keywords

Enzymatic browning
Eriobotrya japonica (Japanese loquat)
Polyphenol oxidase
Prunus persica (peach)
Pseudocydonia sinensis (Chinese quince)
Pyrus communis (pear)
Pyrus pyrifolia (Japanese pear)
Rosaceae

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/S0031-9422(98)00633-5

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@article{39d0b399fc3c418e98ba5da50869f949,

title = "Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees",

abstract = "An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.",

keywords = "Enzymatic browning, Eriobotrya japonica (Japanese loquat), Polyphenol oxidase, Prunus persica (peach), Pseudocydonia sinensis (Chinese quince), Pyrus communis (pear), Pyrus pyrifolia (Japanese pear), Rosaceae",

author = "Miyoshi Haruta and Masatsune Murata and Hiroshi Kadokura and Seiichi Homma",

note = "Funding Information: This study was supported by grant-aid for scientific research from the Ministry of Education, Science and Culture of Japan, No. 09780004 (1997). We thank Dr. Peter Constabel, University of Alberta, for helpful suggestions in preparation of this report.",

year = "1999",

month = mar,

day = "20",

doi = "10.1016/S0031-9422(98)00633-5",

language = "English",

volume = "50",

pages = "1021--1025",

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T1 - Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees

AU - Haruta, Miyoshi

AU - Murata, Masatsune

AU - Kadokura, Hiroshi

AU - Homma, Seiichi

N1 - Funding Information: This study was supported by grant-aid for scientific research from the Ministry of Education, Science and Culture of Japan, No. 09780004 (1997). We thank Dr. Peter Constabel, University of Alberta, for helpful suggestions in preparation of this report.

PY - 1999/3/20

Y1 - 1999/3/20

N2 - An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.

AB - An antibody raised against apple polyphenol oxidase (PPO) cross-reacted with PPOs from Japanese pear (Pyrus pyrifolia), pear (Pyrus communis), peach (Prunus persica), Chinese quince (Pseudocydonia sinensis) and Japanese loquat (Eriobotrya japonica). Core fragments (681 bp) of the corresponding PPO genes were amplified and characterized. The deduced protein sequences showed identities of 85.3 to 97.5%. Chlorogenic acid oxidase activity of these PPOs showed higher activities when assayed at pH 4 than at pH 6. These results indicate that PPOs in Rosaceae plants are structurally and enzymatically similar.

KW - Enzymatic browning

KW - Eriobotrya japonica (Japanese loquat)

KW - Polyphenol oxidase

KW - Prunus persica (peach)

KW - Pseudocydonia sinensis (Chinese quince)

KW - Pyrus communis (pear)

KW - Pyrus pyrifolia (Japanese pear)

KW - Rosaceae

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DO - 10.1016/S0031-9422(98)00633-5

M3 - Article

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AN - SCOPUS:0033103798

SN - 0031-9422

VL - 50

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EP - 1025

JO - Phytochemistry

JF - Phytochemistry

IS - 6

ER -

Immunological and molecular comparison of polyphenol oxidase in Rosaceae fruit trees

Abstract

Keywords

ASJC Scopus subject areas

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