Immunoreactive orexin-A in human plasma

Zenei Arihara; Kazuhiro Takahashi; Osamu Murakami; Kazuhito Totsune; Masahiko Sone; Fumitoshi Satoh; Sadayoshi Ito; Toraichi Mouri

doi:10.1016/S0196-9781(00)00369-7

Immunoreactive orexin-A in human plasma

Zenei Arihara, Kazuhiro Takahashi, Osamu Murakami, Kazuhito Totsune, Masahiko Sone, Fumitoshi Satoh, Sadayoshi Ito, Toraichi Mouri

MED - Health Sciences

Research output: Contribution to journal › Article › peer-review

96 Citations (Scopus)

Abstract

Orexin-A and orexin-B are newly discovered neuropeptides which are implicated in feeding behavior and arousal state. We studied immunoreactive(IR)-orexin-A concentrations in human plasma by radioimmunoassay. IR-orexin-A concentrations in plasma obtained from 17 healthy subjects in the morning were 1.94 ± 0.24 pmol/liter (mean ± SEM). IR-orexin-A levels in the plasma obtained at night were not significantly different from those obtained in the morning in 9 female subjects. The HPLC analysis of the plasma extract showed two immunoreactive peaks; one peak eluting in an identical position to synthetic orexin-A, and one eluting earlier. This study has shown for the first time the presence of orexin-A in human plasma.

Original language	English
Pages (from-to)	139-142
Number of pages	4
Journal	Peptides
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/S0196-9781(00)00369-7
Publication status	Published - 2001

Keywords

Diurnal variation
Orexin-A
Plasma
Radioimmunoassay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0196-9781(00)00369-7

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title = "Immunoreactive orexin-A in human plasma",

abstract = "Orexin-A and orexin-B are newly discovered neuropeptides which are implicated in feeding behavior and arousal state. We studied immunoreactive(IR)-orexin-A concentrations in human plasma by radioimmunoassay. IR-orexin-A concentrations in plasma obtained from 17 healthy subjects in the morning were 1.94 ± 0.24 pmol/liter (mean ± SEM). IR-orexin-A levels in the plasma obtained at night were not significantly different from those obtained in the morning in 9 female subjects. The HPLC analysis of the plasma extract showed two immunoreactive peaks; one peak eluting in an identical position to synthetic orexin-A, and one eluting earlier. This study has shown for the first time the presence of orexin-A in human plasma.",

keywords = "Diurnal variation, Orexin-A, Plasma, Radioimmunoassay",

author = "Zenei Arihara and Kazuhiro Takahashi and Osamu Murakami and Kazuhito Totsune and Masahiko Sone and Fumitoshi Satoh and Sadayoshi Ito and Toraichi Mouri",

note = "Funding Information: The authors are grateful to Ms Kikuchi for her technical assistance. This study has been supported in part by a Research Grant from the Takeda Science Foundation (to K. To.), by Research Grants for the Renal Anemia Research (to K. To. and to K. Ta), by the Mochida Memorial Foundation for Medical and Pharmaceutical Research (to K.Ta.), and a Grant-in-Aid for Scientific Research on Priority Areas (A) (to K. Ta.) from the Ministry of Education, Science, Sports and Culture, Japan.",

year = "2001",

doi = "10.1016/S0196-9781(00)00369-7",

language = "English",

volume = "22",

pages = "139--142",

journal = "Peptides",

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AU - Arihara, Zenei

AU - Takahashi, Kazuhiro

AU - Murakami, Osamu

AU - Totsune, Kazuhito

AU - Sone, Masahiko

AU - Satoh, Fumitoshi

AU - Ito, Sadayoshi

AU - Mouri, Toraichi

N1 - Funding Information: The authors are grateful to Ms Kikuchi for her technical assistance. This study has been supported in part by a Research Grant from the Takeda Science Foundation (to K. To.), by Research Grants for the Renal Anemia Research (to K. To. and to K. Ta), by the Mochida Memorial Foundation for Medical and Pharmaceutical Research (to K.Ta.), and a Grant-in-Aid for Scientific Research on Priority Areas (A) (to K. Ta.) from the Ministry of Education, Science, Sports and Culture, Japan.

PY - 2001

Y1 - 2001

N2 - Orexin-A and orexin-B are newly discovered neuropeptides which are implicated in feeding behavior and arousal state. We studied immunoreactive(IR)-orexin-A concentrations in human plasma by radioimmunoassay. IR-orexin-A concentrations in plasma obtained from 17 healthy subjects in the morning were 1.94 ± 0.24 pmol/liter (mean ± SEM). IR-orexin-A levels in the plasma obtained at night were not significantly different from those obtained in the morning in 9 female subjects. The HPLC analysis of the plasma extract showed two immunoreactive peaks; one peak eluting in an identical position to synthetic orexin-A, and one eluting earlier. This study has shown for the first time the presence of orexin-A in human plasma.

AB - Orexin-A and orexin-B are newly discovered neuropeptides which are implicated in feeding behavior and arousal state. We studied immunoreactive(IR)-orexin-A concentrations in human plasma by radioimmunoassay. IR-orexin-A concentrations in plasma obtained from 17 healthy subjects in the morning were 1.94 ± 0.24 pmol/liter (mean ± SEM). IR-orexin-A levels in the plasma obtained at night were not significantly different from those obtained in the morning in 9 female subjects. The HPLC analysis of the plasma extract showed two immunoreactive peaks; one peak eluting in an identical position to synthetic orexin-A, and one eluting earlier. This study has shown for the first time the presence of orexin-A in human plasma.

KW - Diurnal variation

KW - Orexin-A

KW - Plasma

KW - Radioimmunoassay

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Immunoreactive orexin-A in human plasma

Abstract

Keywords

UN SDGs

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