TY - JOUR
T1 - Impairment of pachytene spermatogenesis in Dmrt7 deficient mice, possibly causing meiotic arrest
AU - Date, Shiori
AU - Nozawa, Osamu
AU - Inoue, Hiroaki
AU - Hidema, Shizu
AU - Nishimori, Katsuhiko
N1 - Funding Information:
We are particularly grateful to Dr. Yuki Takada and Dr. Haruhiko Koseki (RIKEN Research Center for Allergy and Immunology) for advice and help in the meiotic chromosome spread procedure. We thank Dr. Tatsuyuki Takada (Ritsumeikan University) for anti-γH2AX antibody and Dr. Shinichiro Chuma (Kyoto University) for the anti-Scp3 antibody. This work was supported in part by Grant-in-Aid for Scientific Research on Priority Areas, Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2012
Y1 - 2012
N2 - Although Dmrt7 has been reported to be essential for male spermatogenesis, the molecular mechanism underlying pachytene spermatogenesis by Dmrt7 is not known. In the present study, by detailed analysis of Dmrt7 protein distribution in spermatocytes in the first wave of spermatogenesis, we clarified the profile of Dmrt7 expression and localization in pachytene spermatogenesis. Dmrt7-deficient spermatocytes were arrested in the pachytene stage, followed by apoptosis. We analyzed to determine whether every event in the spermatogenesis at the Dmrt7-deficient mice progressed normally, because in several gene knockout mice with spermatogenic arrest described in the previous reports impairments of these events often appeared. Mutant mice showed normal synapsis and XY body formation, while impairment of meiotic sex chromosome inactivation (MSCI), decreased expression of backup genes, and increased expression of retrotransposons indicated incomplete meiotic recombination.
AB - Although Dmrt7 has been reported to be essential for male spermatogenesis, the molecular mechanism underlying pachytene spermatogenesis by Dmrt7 is not known. In the present study, by detailed analysis of Dmrt7 protein distribution in spermatocytes in the first wave of spermatogenesis, we clarified the profile of Dmrt7 expression and localization in pachytene spermatogenesis. Dmrt7-deficient spermatocytes were arrested in the pachytene stage, followed by apoptosis. We analyzed to determine whether every event in the spermatogenesis at the Dmrt7-deficient mice progressed normally, because in several gene knockout mice with spermatogenic arrest described in the previous reports impairments of these events often appeared. Mutant mice showed normal synapsis and XY body formation, while impairment of meiotic sex chromosome inactivation (MSCI), decreased expression of backup genes, and increased expression of retrotransposons indicated incomplete meiotic recombination.
KW - Dmrt
KW - Male infertility
KW - Pachytene
KW - Spermatogenesis
KW - Spermatogenic arrest
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U2 - 10.1271/bbb.120024
DO - 10.1271/bbb.120024
M3 - Article
C2 - 22972322
AN - SCOPUS:84866701533
SN - 0916-8451
VL - 76
SP - 1621
EP - 1626
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 9
ER -