Improved cellulose conversion to bio-hydrogen with thermophilic bacteria and characterization of microbial community in continuous bioreactor

Hongyu Jiang; Samir I. Gadow; Yasumitsu Tanaka; Jun Cheng; Yu You Li

doi:10.1016/j.biombioe.2015.02.010

Improved cellulose conversion to bio-hydrogen with thermophilic bacteria and characterization of microbial community in continuous bioreactor

Hongyu Jiang, Samir I. Gadow, Yasumitsu Tanaka, Jun Cheng, Yu You Li

ENG - Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Thermophilic hydrogen fermentation of cellulose was evaluated by a long term continuous experiment and batch experiments. The continuous experiment was conducted under 55°C using a continuously stirred tank reactor (CSTR) at a hydraulic retention time (HRT) of 10 day. A stable hydrogen yield of 15.4±0.23molkg^-1 of cellulose consumed was maintained for 190 days with acetate and butyrate as the main soluble byproducts. An analysis of the 16S rRNA sequences showed that the hydrogen-producing thermophilic cellulolytic microorganisms (HPTCM) were close to Thermoanaerobacterium thermosaccharolyticum, Clostridium sp. and Enterobacter cloacae. Batch experiment demonstrated that the highest H₂ producing activity was obtained at 55°C and the ultimate hydrogen yield and the metabolic by-products were influenced greatly by temperatures. The effect of temperature variation showed that the activation energy for cellulose and glucose were estimated at 103 and 98.8kJmol^-1, respectively.

Original language	English
Pages (from-to)	57-64
Number of pages	8
Journal	Biomass and Bioenergy
Volume	75
DOIs	https://doi.org/10.1016/j.biombioe.2015.02.010
Publication status	Published - 2015 Apr 1

Keywords

Activity test
Cellulose
Dark fermentation
Microbial community structure
Thermophilic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biombioe.2015.02.010

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title = "Improved cellulose conversion to bio-hydrogen with thermophilic bacteria and characterization of microbial community in continuous bioreactor",

abstract = "Thermophilic hydrogen fermentation of cellulose was evaluated by a long term continuous experiment and batch experiments. The continuous experiment was conducted under 55°C using a continuously stirred tank reactor (CSTR) at a hydraulic retention time (HRT) of 10 day. A stable hydrogen yield of 15.4±0.23molkg-1 of cellulose consumed was maintained for 190 days with acetate and butyrate as the main soluble byproducts. An analysis of the 16S rRNA sequences showed that the hydrogen-producing thermophilic cellulolytic microorganisms (HPTCM) were close to Thermoanaerobacterium thermosaccharolyticum, Clostridium sp. and Enterobacter cloacae. Batch experiment demonstrated that the highest H2 producing activity was obtained at 55°C and the ultimate hydrogen yield and the metabolic by-products were influenced greatly by temperatures. The effect of temperature variation showed that the activation energy for cellulose and glucose were estimated at 103 and 98.8kJmol-1, respectively.",

keywords = "Activity test, Cellulose, Dark fermentation, Microbial community structure, Thermophilic",

author = "Hongyu Jiang and Gadow, {Samir I.} and Yasumitsu Tanaka and Jun Cheng and Li, {Yu You}",

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T1 - Improved cellulose conversion to bio-hydrogen with thermophilic bacteria and characterization of microbial community in continuous bioreactor

AU - Jiang, Hongyu

AU - Gadow, Samir I.

AU - Tanaka, Yasumitsu

AU - Cheng, Jun

AU - Li, Yu You

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Thermophilic hydrogen fermentation of cellulose was evaluated by a long term continuous experiment and batch experiments. The continuous experiment was conducted under 55°C using a continuously stirred tank reactor (CSTR) at a hydraulic retention time (HRT) of 10 day. A stable hydrogen yield of 15.4±0.23molkg-1 of cellulose consumed was maintained for 190 days with acetate and butyrate as the main soluble byproducts. An analysis of the 16S rRNA sequences showed that the hydrogen-producing thermophilic cellulolytic microorganisms (HPTCM) were close to Thermoanaerobacterium thermosaccharolyticum, Clostridium sp. and Enterobacter cloacae. Batch experiment demonstrated that the highest H2 producing activity was obtained at 55°C and the ultimate hydrogen yield and the metabolic by-products were influenced greatly by temperatures. The effect of temperature variation showed that the activation energy for cellulose and glucose were estimated at 103 and 98.8kJmol-1, respectively.

AB - Thermophilic hydrogen fermentation of cellulose was evaluated by a long term continuous experiment and batch experiments. The continuous experiment was conducted under 55°C using a continuously stirred tank reactor (CSTR) at a hydraulic retention time (HRT) of 10 day. A stable hydrogen yield of 15.4±0.23molkg-1 of cellulose consumed was maintained for 190 days with acetate and butyrate as the main soluble byproducts. An analysis of the 16S rRNA sequences showed that the hydrogen-producing thermophilic cellulolytic microorganisms (HPTCM) were close to Thermoanaerobacterium thermosaccharolyticum, Clostridium sp. and Enterobacter cloacae. Batch experiment demonstrated that the highest H2 producing activity was obtained at 55°C and the ultimate hydrogen yield and the metabolic by-products were influenced greatly by temperatures. The effect of temperature variation showed that the activation energy for cellulose and glucose were estimated at 103 and 98.8kJmol-1, respectively.

KW - Activity test

KW - Cellulose

KW - Dark fermentation

KW - Microbial community structure

KW - Thermophilic

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DO - 10.1016/j.biombioe.2015.02.010

M3 - Article

AN - SCOPUS:84923615899

SN - 0961-9534

VL - 75

SP - 57

EP - 64

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

ER -

Improved cellulose conversion to bio-hydrogen with thermophilic bacteria and characterization of microbial community in continuous bioreactor

Abstract

Keywords

UN SDGs

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