Improving the biomass retention and system stability of the anammox EGSB reactor by adding a calcium silicate hydrate functional material

Improving the biomass retention and the sludge system stability to promote the full-scale application of anammox process is the focus of current related research. In this study, a calcium silicate hydrate functional material with calcium-releasing ability and weak alkalinity was used for an enhanced anammox process. In the long-term operation, an increase in the nitrogen removal rate (NRR) from 2.75 to 13.38 gN/L/d was achieved after 50 days of operation, with the abundance of Candidatus Kuenenia increased from 40.1 % to 47.0 %. The anammox activity was strengthened from 0.089 to 0.55 gN/gVSS/d over 50 days, with a growth rate being fitted at 0.0310 d⁻¹. The resilience of the EGSB anammox system after inhibitions was investigated by substrate shock and low pH shock in long-term operation and batch test. Besides that, the phosphorus removal efficiency of the reactor reached up to 90 % under the positive effect of functional material. The functional material was shown to continuously provide calcium in the long-term for the reaction of hydroxyapatite (HAP) formation, which further improved the granular properties of the sludge and the biomass retention ability of the reactor. The promotion effect of functional material on the sludge granulation and anammox microbes retaining efficiency was the key for a high-resilience anammox EGSB reactor.