TY - JOUR
T1 - In situ control of cellular growth and migration on substrates using microelectrodes
AU - Kaji, Hirokazu
AU - Tsukidate, Kazuto
AU - Matsue, Tomokazu
AU - Nishizawa, Matsuhiko
PY - 2004/11/24
Y1 - 2004/11/24
N2 - We describe an electrochemical method to direct the growth and migration of mammalian cells on a substrate during cultivation in situ. Exposing the albumin-coated substrate to an oxidizing agent, hypobromous acid, electrochemically generated at the tip of the scanning microelectrode, locally switched the substrate from cytophobic to cell-adhesive. This transformation generated the formation of cellular micropatterns. Since the concentration of the oxidizing agent required for the surface processing did not cause significant damage to the cell cultures, we were able to direct in situ cellular proliferation and migration by drawing adhesive micropatterns over the preexisting cellular pattern.
AB - We describe an electrochemical method to direct the growth and migration of mammalian cells on a substrate during cultivation in situ. Exposing the albumin-coated substrate to an oxidizing agent, hypobromous acid, electrochemically generated at the tip of the scanning microelectrode, locally switched the substrate from cytophobic to cell-adhesive. This transformation generated the formation of cellular micropatterns. Since the concentration of the oxidizing agent required for the surface processing did not cause significant damage to the cell cultures, we were able to direct in situ cellular proliferation and migration by drawing adhesive micropatterns over the preexisting cellular pattern.
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U2 - 10.1021/ja045702t
DO - 10.1021/ja045702t
M3 - Article
AN - SCOPUS:9344243556
SN - 0002-7863
VL - 126
SP - 15026
EP - 15027
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 46
ER -