In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

Hideaki Yamamoto; Kazunori Okano; Takanori Demura; Yoichiroh Hosokawa; Hiroshi Masuhara; Takashi Tanii; Shun Nakamura

doi:10.1063/1.3651291

In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

Hideaki Yamamoto, Kazunori Okano, Takanori Demura, Yoichiroh Hosokawa, Hiroshi Masuhara, Takashi Tanii, Shun Nakamura

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro.

Original language	English
Article number	163701
Journal	Applied Physics Letters
Volume	99
Issue number	16
DOIs	https://doi.org/10.1063/1.3651291
Publication status	Published - 2011 Oct 17
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.3651291

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abstract = "In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro.",

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AU - Yamamoto, Hideaki

AU - Okano, Kazunori

AU - Demura, Takanori

AU - Hosokawa, Yoichiroh

AU - Masuhara, Hiroshi

AU - Tanii, Takashi

AU - Nakamura, Shun

N1 - Funding Information: The authors acknowledge Ms. Mie Matsubara and Ms. Sayaka Sakaguchi of the Nara Institute of Science and Technology for setting up equipment. This work was supported by the Research Fellowships for Young Scientists (HY) and by the Grant-in-Aid for Scientific Research (B) (No. 20310075) (KO) from the Japan Society for the Promotion of Science.

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AB - In-situ guidance of neuronal processes (neurites) is demonstrated by applying wet femtosecond-laser processing to an organosilane self-assembled monolayer (SAM) template. By scanning focused laser beam between cell adhesion sites, on which primary neurons adhered and extended their neurites, we succeeded in guiding the neurites along the laser-scanning line. This guidance was accomplished by multiphoton laser ablation of cytophobic SAM layer and subsequent adsorption of cell adhesion molecule, laminin, onto the ablated region. This technique allows us to arbitrarily design neuronal networks in vitro.

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In-situ guidance of individual neuronal processes by wet femtosecond-laser processing of self-assembled monolayers

Abstract

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