In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy

Ko Ichiro Miyamoto; Parida Yamada; Ryo Taro Yamaguchi; Takami Muto; Ayumi Hirano; Yasuo Kimura; Michio Niwano; Hiroko Isoda

doi:10.1007/s10616-007-9111-2

In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy

Ko Ichiro Miyamoto, Parida Yamada, Ryo Taro Yamaguchi, Takami Muto, Ayumi Hirano, Yasuo Kimura, Michio Niwano, Hiroko Isoda

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16 Citations (Scopus)

Abstract

In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37°C and a humidified gas mixture containing 5% CO₂ was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm^-1) and also by the absorption intensities of CH _x bands (2,700-3,100 cm^-1). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.

Original language	English
Pages (from-to)	143-149
Number of pages	7
Journal	Cytotechnology
Volume	55
Issue number	2-3
DOIs	https://doi.org/10.1007/s10616-007-9111-2
Publication status	Published - 2007 Dec

Keywords

Cell metabolism
Human breast cell line MCF-7
In situ observation
Infrared absorption spectroscopy
Si prisms

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10.1007/s10616-007-9111-2

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title = "In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy",

abstract = "In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37°C and a humidified gas mixture containing 5% CO2 was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm-1) and also by the absorption intensities of CH x bands (2,700-3,100 cm-1). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.",

keywords = "Cell metabolism, Human breast cell line MCF-7, In situ observation, Infrared absorption spectroscopy, Si prisms",

author = "Miyamoto, {Ko Ichiro} and Parida Yamada and Yamaguchi, {Ryo Taro} and Takami Muto and Ayumi Hirano and Yasuo Kimura and Michio Niwano and Hiroko Isoda",

note = "Funding Information: Acknowledgement Part of this work was supported by Grants-in-Aid for Scientific Research (Grant No. 17206004) from the Japan Society for the Promotion of Science (JSPS).",

year = "2007",

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doi = "10.1007/s10616-007-9111-2",

language = "English",

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T1 - In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy

AU - Miyamoto, Ko Ichiro

AU - Yamada, Parida

AU - Yamaguchi, Ryo Taro

AU - Muto, Takami

AU - Hirano, Ayumi

AU - Kimura, Yasuo

AU - Niwano, Michio

AU - Isoda, Hiroko

N1 - Funding Information: Acknowledgement Part of this work was supported by Grants-in-Aid for Scientific Research (Grant No. 17206004) from the Japan Society for the Promotion of Science (JSPS).

PY - 2007/12

Y1 - 2007/12

N2 - In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37°C and a humidified gas mixture containing 5% CO2 was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm-1) and also by the absorption intensities of CH x bands (2,700-3,100 cm-1). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.

AB - In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37°C and a humidified gas mixture containing 5% CO2 was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm-1) and also by the absorption intensities of CH x bands (2,700-3,100 cm-1). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.

KW - Cell metabolism

KW - Human breast cell line MCF-7

KW - In situ observation

KW - Infrared absorption spectroscopy

KW - Si prisms

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JO - Cytotechnology

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In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy

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