TY - JOUR
T1 - In vitro evaluation of Ag-containing calcium phosphates
T2 - Effectiveness of Ag-incorporated β-tricalcium phosphate
AU - Gokcekaya, Ozkan
AU - Ueda, Kyosuke
AU - Ogasawara, Kouetsu
AU - Kanetaka, Hiroyasu
AU - Narushima, Takayuki
N1 - Funding Information:
This study was financially supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) under Contract No. 201500660 and the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan under Contract Nos: 25249094 and 26709049.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Development of bioceramics with antibacterial activity and without cytotoxicity would be beneficial for preventing infection associated with implants. This study aimed to capitalize on the antibacterial properties of silver (Ag) incorporated in or coexisting in metallic form with calcium phosphates (CaPs). The in vitro dissolution behavior, antibacterial activity, and cytotoxicity of Ag-containing CaPs with different phase fractions of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) were evaluated. The antibacterial activity of Ag-containing CaPs depended on the main phase of CaP, the chemical state of Ag, and the amount of incorporated Ag. Superior antibacterial activity was obtained from sustained release of Ag ions through continuous dissolution of Ag-incorporated β-TCP compared to that obtained for HA coexisting with metallic Ag particles. Ag-containing CaPs did not exhibit any toxic effect on V79 fibroblasts. Thus, these results demonstrated the effectiveness of Ag-incorporated β-TCP in preventing infection, with respect to long-term applications.
AB - Development of bioceramics with antibacterial activity and without cytotoxicity would be beneficial for preventing infection associated with implants. This study aimed to capitalize on the antibacterial properties of silver (Ag) incorporated in or coexisting in metallic form with calcium phosphates (CaPs). The in vitro dissolution behavior, antibacterial activity, and cytotoxicity of Ag-containing CaPs with different phase fractions of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) were evaluated. The antibacterial activity of Ag-containing CaPs depended on the main phase of CaP, the chemical state of Ag, and the amount of incorporated Ag. Superior antibacterial activity was obtained from sustained release of Ag ions through continuous dissolution of Ag-incorporated β-TCP compared to that obtained for HA coexisting with metallic Ag particles. Ag-containing CaPs did not exhibit any toxic effect on V79 fibroblasts. Thus, these results demonstrated the effectiveness of Ag-incorporated β-TCP in preventing infection, with respect to long-term applications.
KW - Antibacterial activity
KW - Calcium phosphate
KW - Cytotoxicity
KW - In vitro dissolution
KW - Silver
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U2 - 10.1016/j.msec.2017.02.059
DO - 10.1016/j.msec.2017.02.059
M3 - Article
C2 - 28415548
AN - SCOPUS:85014414864
SN - 0928-4931
VL - 75
SP - 926
EP - 933
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
ER -