In vivo optical recording of brain interneuron activities from a drosophila male on a treadmill

Soh Kohatsu, Masayuki Koganezawa, Daisuke Yamamoto

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Recent development of genetically encoded calcium indicators (GECIs) allows us to directly monitor neural activities in in vivo preparations from various organisms, enabling the exploration of neural substrates for complex behaviors. As a showcase for such renovated neuroethology with GECIs, we describe a novel method to record optically interneuron activities from the brain of a tethered male fruit fly Drosophila melanogaster when contact chemosensory stimulation is given to his foreleg. In this protocol, the recording is made on the preparation in which a fly is put on an air-supported styrofoam ball, keeping the moving fly in position to allow monitoring of the activity of central neurons from the fly whose movement is minimally restricted. This protocol is applicable to the analysis of other behaviors induced by stimuli of different modalities with small modifications.

Original languageEnglish
Title of host publicationGenetically Encoded Functional Indicators
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781627030137
Publication statusPublished - 2012

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045


  • Calcium imaging
  • Courtship
  • Drosophila
  • Fruitless
  • Yellow Cameleon


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