Incorporation of impurity to a tetragonal lysozyme crystal

Kazuo Kurihara; Satoru Miyashita; Gen Sazaki; Toshitaka Nakada; Stephen D. Durbin; Hiroshi Komatsu; Tetsuhiko Ohba; Kazuo Ohki

doi:10.1016/S0022-0248(98)00872-0

Incorporation of impurity to a tetragonal lysozyme crystal

Kazuo Kurihara, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Stephen D. Durbin, Hiroshi Komatsu, Tetsuhiko Ohba, Kazuo Ohki

SCI - Physics

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Concentration of a phosphor-labeled impurity (ovalbumin) incorporated into protein (hen egg white lysozyme) crystals during growth was measured by fluorescence.This technique enabled us to measure the local impurity concentration in a crystal quantitatively. Impurity concentration increased with growth rate, which could not be explained by two conventional models (equilibrium adsorption model and Burton-Prim-Slichter model); a modified model is proposed. Impurity concentration also increased with the pH of the solution. This result is discussed considering the electrostatic interaction between the impurity and the crystallizing species.

Original language	English
Pages (from-to)	285-290
Number of pages	6
Journal	Journal of Crystal Growth
Volume	196
Issue number	2-4
DOIs	https://doi.org/10.1016/S0022-0248(98)00872-0
Publication status	Published - 1999 Jan 15

Keywords

Crystal growth
Fluorescence
Impurity
Incorporation
Lysozyme
Ovalbumin

ASJC Scopus subject areas

Condensed Matter Physics
Inorganic Chemistry
Materials Chemistry

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10.1016/S0022-0248(98)00872-0

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title = "Incorporation of impurity to a tetragonal lysozyme crystal",

abstract = "Concentration of a phosphor-labeled impurity (ovalbumin) incorporated into protein (hen egg white lysozyme) crystals during growth was measured by fluorescence.This technique enabled us to measure the local impurity concentration in a crystal quantitatively. Impurity concentration increased with growth rate, which could not be explained by two conventional models (equilibrium adsorption model and Burton-Prim-Slichter model); a modified model is proposed. Impurity concentration also increased with the pH of the solution. This result is discussed considering the electrostatic interaction between the impurity and the crystallizing species.",

keywords = "Crystal growth, Fluorescence, Impurity, Incorporation, Lysozyme, Ovalbumin",

author = "Kazuo Kurihara and Satoru Miyashita and Gen Sazaki and Toshitaka Nakada and Durbin, {Stephen D.} and Hiroshi Komatsu and Tetsuhiko Ohba and Kazuo Ohki",

note = "Funding Information: The authors would like to thank Nikon Instech Co. for the loan of an optical microscope. They are also grateful for partial support by two Grants-in-Aid (No.07780558 and No. 09044055) of Scientific Research of the Ministry of Education, Science and Culture Japan, as well as the support by a fund from Sumitomo Foundation. This study was carried out as a part of “Space Utilization Frontiers Joint Research Projects” promoted by NASDA and Japan Space Utilization Promotion Center. In this work, NIH-Image was used for intensity measurement of fluorescence images.",

year = "1999",

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doi = "10.1016/S0022-0248(98)00872-0",

language = "English",

volume = "196",

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T1 - Incorporation of impurity to a tetragonal lysozyme crystal

AU - Kurihara, Kazuo

AU - Miyashita, Satoru

AU - Sazaki, Gen

AU - Nakada, Toshitaka

AU - Durbin, Stephen D.

AU - Komatsu, Hiroshi

AU - Ohba, Tetsuhiko

AU - Ohki, Kazuo

N1 - Funding Information: The authors would like to thank Nikon Instech Co. for the loan of an optical microscope. They are also grateful for partial support by two Grants-in-Aid (No.07780558 and No. 09044055) of Scientific Research of the Ministry of Education, Science and Culture Japan, as well as the support by a fund from Sumitomo Foundation. This study was carried out as a part of “Space Utilization Frontiers Joint Research Projects” promoted by NASDA and Japan Space Utilization Promotion Center. In this work, NIH-Image was used for intensity measurement of fluorescence images.

PY - 1999/1/15

Y1 - 1999/1/15

N2 - Concentration of a phosphor-labeled impurity (ovalbumin) incorporated into protein (hen egg white lysozyme) crystals during growth was measured by fluorescence.This technique enabled us to measure the local impurity concentration in a crystal quantitatively. Impurity concentration increased with growth rate, which could not be explained by two conventional models (equilibrium adsorption model and Burton-Prim-Slichter model); a modified model is proposed. Impurity concentration also increased with the pH of the solution. This result is discussed considering the electrostatic interaction between the impurity and the crystallizing species.

AB - Concentration of a phosphor-labeled impurity (ovalbumin) incorporated into protein (hen egg white lysozyme) crystals during growth was measured by fluorescence.This technique enabled us to measure the local impurity concentration in a crystal quantitatively. Impurity concentration increased with growth rate, which could not be explained by two conventional models (equilibrium adsorption model and Burton-Prim-Slichter model); a modified model is proposed. Impurity concentration also increased with the pH of the solution. This result is discussed considering the electrostatic interaction between the impurity and the crystallizing species.

KW - Crystal growth

KW - Fluorescence

KW - Impurity

KW - Incorporation

KW - Lysozyme

KW - Ovalbumin

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DO - 10.1016/S0022-0248(98)00872-0

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SN - 0022-0248

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SP - 285

EP - 290

JO - Journal of Crystal Growth

JF - Journal of Crystal Growth

IS - 2-4

ER -

Incorporation of impurity to a tetragonal lysozyme crystal

Abstract

Keywords

ASJC Scopus subject areas

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