TY - JOUR
T1 - Increased expression of (pro)renin receptor in the remnant kidneys of 5/6 nephrectomized rats
AU - Hirose, Takuo
AU - Mori, Nobuyoshi
AU - Totsune, Kazuhito
AU - Morimoto, Ryo
AU - Maejima, Takahiro
AU - Kawamura, Takuya
AU - Metoki, Hirohito
AU - Asayama, Kei
AU - Kikuya, Masahiro
AU - Ohkubo, Takayoshi
AU - Kohzuki, Masahiro
AU - Takahashi, Kazuhiro
AU - Imai, Yutaka
N1 - Funding Information:
This study was supported in part by Grants-in-aid for Scientific Research (C) from the Ministry of Education, Science, Sports and Culture of Japan , by a Research Grant from the Takeda Science Foundation (2005), by Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) fellows , and by the Tohoku University 21st Century Center of Excellence Program “Comprehensive Research and Education Center for Planning of Drug Development and Clinical Evaluation (CRESCENDO)”.
PY - 2010/1/8
Y1 - 2010/1/8
N2 - Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, is associated with renal organ damage. However, there is little information regarding the regulation of (P)RR expression in various pathophysiological conditions. We therefore examined the expression of (P)RR in the remnant kidneys of rats with renal mass ablation due to 5/6 nephrectomy by quantitative RT-PCR, Western blot analysis and immunohistochemistry. Expression levels of (P)RR mRNA were significantly increased in the remnant kidneys at day 56 after nephrectomy, when compared with sham operation (about 1.6-fold, P = 0.001). Western blot analysis showed that expression levels of (P)RR protein were greatly increased in the remnant kidneys at day 56, compared with sham operation (about 7.9-fold, P = 0.02). The renal tubular cells were immunostained with anti-(P)RR antibody in both 5/6 nephrectomized rats and sham operated rats. The glomeruli were sporadically immunostained in 5/6 nephrectomized rats, but not in sham operated rats. These findings indicate that the intra-renal (P)RR expression is increased in the remnant kidneys of 5/6 nephrectomized rats, and suggest that (P)RR may contribute to the renal injury.
AB - Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, is associated with renal organ damage. However, there is little information regarding the regulation of (P)RR expression in various pathophysiological conditions. We therefore examined the expression of (P)RR in the remnant kidneys of rats with renal mass ablation due to 5/6 nephrectomy by quantitative RT-PCR, Western blot analysis and immunohistochemistry. Expression levels of (P)RR mRNA were significantly increased in the remnant kidneys at day 56 after nephrectomy, when compared with sham operation (about 1.6-fold, P = 0.001). Western blot analysis showed that expression levels of (P)RR protein were greatly increased in the remnant kidneys at day 56, compared with sham operation (about 7.9-fold, P = 0.02). The renal tubular cells were immunostained with anti-(P)RR antibody in both 5/6 nephrectomized rats and sham operated rats. The glomeruli were sporadically immunostained in 5/6 nephrectomized rats, but not in sham operated rats. These findings indicate that the intra-renal (P)RR expression is increased in the remnant kidneys of 5/6 nephrectomized rats, and suggest that (P)RR may contribute to the renal injury.
KW - Immunohistochemistry
KW - Organ damage
KW - Renin-angiotensin system
KW - RT-PCR
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=71349085194&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=71349085194&partnerID=8YFLogxK
U2 - 10.1016/j.regpep.2009.11.006
DO - 10.1016/j.regpep.2009.11.006
M3 - Article
C2 - 19896985
AN - SCOPUS:71349085194
SN - 0167-0115
VL - 159
SP - 93
EP - 99
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 1-3
ER -