Increased oxidative stress in mouse kidneys with unilateral ureteral obstruction

Noritaka Kawada, Toshiki Moriyama, Akio Ando, Megumu Fukunaga, Toshio Miyata, Kiyoshi Kurokawa, Enyu Imai, Masatsugu Hori

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)


Background. Unilateral ureteral obstruction (UUO) is a well-established experimental model of renal injury leading to interstitial fibrosis. The molecular and cellular mechanism(s) of interstitial fibrosis in UUO kidney is beginning to be elucidated. Oxidative stress has been implicated in the pathogenesis of various forms of renal injury; however, little is known about its involvement in the setting of ureteral obstruction. Methods. To investigate the possible involvement of oxidative stress in the obstructive nephropathy, we studied the occurrence and distribution of N(ε)- carboxymethyl-lysine (CML) in the kidneys after ureteral obstruction. CML is an integrative biomarker of the cumulative protein damage induced by glycoxidation. Heme oxygenase-1 (HO-1) mRNA and protein expression, which is a sensitive and reliable indicator of oxidative stress, were also examined. Results. CML immunoreactivity was found in the interstitium of UUO kidneys 10 days after the onset ureteral obstruction. HO-1 mRNA was up-regulated as early as 12 hours after ureteral obstruction. HO-1 immunoreactivity was observed in the periglomerular and peritubular interstitium two days after ureteral obstruction. Conclusions. These results strongly suggested the presence of increased oxidative stress in the interstitium of UUO kidneys. The oxidative stress and the formation of various kind of biological active oxidative products in the interstitium are supposed to play significant roles in UUO kidney.

Original languageEnglish
Pages (from-to)1004-1013
Number of pages10
JournalKidney international
Issue number3
Publication statusPublished - 1999
Externally publishedYes


  • Advanced glycation end product
  • Heme oxygenase-1
  • Interstitial fibrosis
  • Renal injury
  • UUO

ASJC Scopus subject areas

  • Nephrology


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