TY - JOUR
T1 - Increased tolerance to salt- and water-deficit stress in transgenic lettuce (Lactuca sativa L.) by constitutive expression of LEA
AU - Park, Byong Jin
AU - Liu, Zaochang
AU - Kanno, Akira
AU - Kameya, Toshiaki
N1 - Funding Information:
The authors are grateful to Dr Takahata, Faculty of Agriculture, Iwate University of Japan, for gift of LEA cDNA clone. This work was partially supported by a grant (Code 03DJ14015) from Science and Technology Committee of Shanghai Municipal Administration, China. Byong-Jin Park was supported by a Postgraduate Studentship from Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2005/2
Y1 - 2005/2
N2 - A late embryogenesis abundant (LEA) protein gene, ME-leaN4, from rape (Brassica napus) was successfully introduced into lettuce (Lactuca sativa L.) using Agrobacterium tumefaciens-mediated transformation. Infection by Agrobacterium strain EHA101 containing the binary vector pIG121-LEA was applied. Six independent transgenic lettuce plants were generated as a result. Transgenic lettuce demonstrated enhanced growth ability as compared to non-transformed control plants under salt-stress and water-deficit stress conditions. After 10-day growth under 100 mM NaCl condition in the hydroponics, average plant length and fresh weight of transgenic lettuce were 2.8 cm and 2.5 g plant-1, while control plants were only 0.2 cm and 0.3 g plant -1, respectively. The increased tolerance was also reflected by delayed wilting of leaves caused by water-deficit stress. These results suggest that growth characteristics were improved in transgenic lettuce plants constitutively expressing the rape LEA gene in response to salt- and water-deficit stress.
AB - A late embryogenesis abundant (LEA) protein gene, ME-leaN4, from rape (Brassica napus) was successfully introduced into lettuce (Lactuca sativa L.) using Agrobacterium tumefaciens-mediated transformation. Infection by Agrobacterium strain EHA101 containing the binary vector pIG121-LEA was applied. Six independent transgenic lettuce plants were generated as a result. Transgenic lettuce demonstrated enhanced growth ability as compared to non-transformed control plants under salt-stress and water-deficit stress conditions. After 10-day growth under 100 mM NaCl condition in the hydroponics, average plant length and fresh weight of transgenic lettuce were 2.8 cm and 2.5 g plant-1, while control plants were only 0.2 cm and 0.3 g plant -1, respectively. The increased tolerance was also reflected by delayed wilting of leaves caused by water-deficit stress. These results suggest that growth characteristics were improved in transgenic lettuce plants constitutively expressing the rape LEA gene in response to salt- and water-deficit stress.
KW - Agrobacterium-mediated transformation
KW - LEA (late embryogenesis abundant)
KW - Lettuce
KW - Salt-tolerance
KW - Water-deficit tolerance
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U2 - 10.1007/s10725-004-7924-y
DO - 10.1007/s10725-004-7924-y
M3 - Article
AN - SCOPUS:19944398475
SN - 0167-6903
VL - 45
SP - 165
EP - 171
JO - Plant Growth Regulation
JF - Plant Growth Regulation
IS - 2
ER -