TY - JOUR
T1 - Increasing cell–device adherence using cultured insect cells for receptor-based biosensors
AU - Terutsuki, Daigo
AU - Mitsuno, Hidefumi
AU - Sakurai, Takeshi
AU - Okamoto, Yuki
AU - Tixier-Mita, Agnès
AU - Toshiyoshi, Hiroshi
AU - Mita, Yoshio
AU - Kanzaki, Ryohei
N1 - Funding Information:
D.T. was supported by the Tateisi Science and Technology Foundation, 2016 Research Grant (C). D.T., H.M., T.S. and R.K. were supported by the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry for establishment of the Sf21 cell lines. Acknowledgements. This research was supported by VLSI Design and Education Center (VDEC), The University of Tokyo, with the collaboration of Cadence Corporation. The authors thank JEOL, Ltd and Hitachi UTokyo Laboratory for helpful discussions and for supporting the SEM observations, and Ms Eri Kuroda for her technical support. The GCaMP6s gene was kindly provided by Dr Douglas S. Kim with Janelia Farm Research Campus, Howard Hughes Medical Institute, VA, USA. Bombykal was kindly provided by Dr Shigeru Matsuyama with Tsukuba University, Ibaraki, Japan.
Funding Information:
Ethics. All genetic experiments in this research followed life science research ethics and safety of the University of Tokyo. Data accessibility. All data used in this research are included in the figures, the table and the electronic supplementary material. Authors’ contributions. D.T., H.M., T.S. and R.K. designed the study; D.T. carried out laboratory work, conducted image analysis and statistical analysis; D.T. wrote the manuscript; Y.O. supported device and specimen preparations; H.M., T.S., A.T.-M., H.T., Y.M. and R.K. helped draft the manuscript. All authors gave final approval for publication. Competing interests. We have no competing interests. Funding. D.T. was supported by the Tateisi Science and Technology Foundation, 2016 Research Grant (C). D.T., H.M., T.S. and R.K. were supported by the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry for establishment of the Sf21 cell lines. Acknowledgements. This research was supported by VLSI Design and Education Center (VDEC), The University of Tokyo, with the collaboration of Cadence Corporation. The authors thank JEOL, Ltd and Hitachi UTokyo Laboratory for helpful discussions and for supporting the SEM observations, and Ms Eri Kuroda for her technical support. The GCaMP6s gene was kindly provided by Dr Douglas S. Kim with Janelia Farm Research Campus, Howard Hughes Medical Institute, VA, USA. Bombykal was kindly provided by Dr Shigeru Matsuyama with Tsukuba University, Ibaraki, Japan.
Publisher Copyright:
© 2018 The Authors.
PY - 2018/3/21
Y1 - 2018/3/21
N2 - Field-effect transistor (FET)-based biosensors have a wide range of applications, and a bio-FET odorant sensor, based on insect (Sf21) cells expressing insect odorant receptors (ORs) with sensitivity and selectivity, has emerged. To fully realize the practical application of bio-FET odorant sensors, knowledge of the cell–device interface for efficient signal transfer, and a reliable and low-cost measurement system using the commercial complementary metal-oxide semiconductor (CMOS) foundry process, will be indispensable. However, the interfaces between Sf21 cells and sensor devices are largely unknown, and electrode materials used in the commercial CMOS foundry process are generally limited to aluminium, which is reportedly toxic to cells. In this study, we investigated Sf21 cell–device interfaces by developing cross-sectional specimens. Calcium imaging of Sf21 cells expressing insect ORs was used to verify the functions of Sf21 cells as odorant sensor elements on the electrode materials. We found that the cell–device interface was approximately 10 nm wide on average, suggesting that the adhesion mechanism of Sf21 cells may differ from that of other cells. These results will help to construct accurate signal detection from expressed insect ORs using FETs.
AB - Field-effect transistor (FET)-based biosensors have a wide range of applications, and a bio-FET odorant sensor, based on insect (Sf21) cells expressing insect odorant receptors (ORs) with sensitivity and selectivity, has emerged. To fully realize the practical application of bio-FET odorant sensors, knowledge of the cell–device interface for efficient signal transfer, and a reliable and low-cost measurement system using the commercial complementary metal-oxide semiconductor (CMOS) foundry process, will be indispensable. However, the interfaces between Sf21 cells and sensor devices are largely unknown, and electrode materials used in the commercial CMOS foundry process are generally limited to aluminium, which is reportedly toxic to cells. In this study, we investigated Sf21 cell–device interfaces by developing cross-sectional specimens. Calcium imaging of Sf21 cells expressing insect ORs was used to verify the functions of Sf21 cells as odorant sensor elements on the electrode materials. We found that the cell–device interface was approximately 10 nm wide on average, suggesting that the adhesion mechanism of Sf21 cells may differ from that of other cells. These results will help to construct accurate signal detection from expressed insect ORs using FETs.
KW - Cell adhesion
KW - Cell-Electronic interface
KW - Cross-section polisher
KW - Field-effect transistor
KW - Odorant sensor
KW - Sf21 insect cell
UR - http://www.scopus.com/inward/record.url?scp=85044281016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85044281016&partnerID=8YFLogxK
U2 - 10.1098/rsos.172366
DO - 10.1098/rsos.172366
M3 - Article
AN - SCOPUS:85044281016
SN - 2054-5703
VL - 5
JO - Royal Society Open Science
JF - Royal Society Open Science
IS - 3
M1 - 172366
ER -