Incubation type planar patch clamp biosensor: Basic performances

Tsuneo Urisu; Hidetaka Uno; Zhi Hong Wang; Senthil Kumar Obuliraj; Noriko Takada; Masaki Aoyama; Mitsukazu Suzui; Toshifumi Asano; Toru Ishizuka; Hiromu Yawo

Incubation type planar patch clamp biosensor: Basic performances

Tsuneo Urisu, Hidetaka Uno, Zhi Hong Wang, Senthil Kumar Obuliraj, Noriko Takada, Masaki Aoyama, Mitsukazu Suzui, Toshifumi Asano, Toru Ishizuka, Hiromu Yawo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.

Original language	English
Title of host publication	BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
Pages	143-148
Number of pages	6
Publication status	Published - 2012
Event	International Conference on Biomedical Electronics and Devices, BIODEVICES 2012 - Vilamoura, Algarve, Portugal Duration: 2012 Feb 1 → 2012 Feb 4

Publication series

Name	BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

Other

Other	International Conference on Biomedical Electronics and Devices, BIODEVICES 2012
Country/Territory	Portugal
City	Vilamoura, Algarve
Period	12/2/1 → 12/2/4

Keywords

Biosensor
HEK293
Ionchannel
PMMA
Planar patch clamp
TRPV1

ASJC Scopus subject areas

Biomedical Engineering
Electrical and Electronic Engineering

Cite this

Urisu, T., Uno, H., Wang, Z. H., Obuliraj, S. K., Takada, N., Aoyama, M., Suzui, M., Asano, T., Ishizuka, T., & Yawo, H. (2012). Incubation type planar patch clamp biosensor: Basic performances. In BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices (pp. 143-148). (BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices).

Incubation type planar patch clamp biosensor : Basic performances. / Urisu, Tsuneo; Uno, Hidetaka; Wang, Zhi Hong et al.

BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. 2012. p. 143-148 (BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Urisu, T, Uno, H, Wang, ZH, Obuliraj, SK, Takada, N, Aoyama, M, Suzui, M, Asano, T, Ishizuka, T & Yawo, H 2012, Incubation type planar patch clamp biosensor: Basic performances. in BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices, pp. 143-148, International Conference on Biomedical Electronics and Devices, BIODEVICES 2012, Vilamoura, Algarve, Portugal, 12/2/1.

@inproceedings{2dc41c1fac7d4edda854be24bf4f620b,

title = "Incubation type planar patch clamp biosensor: Basic performances",

abstract = "The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.",

keywords = "Biosensor, HEK293, Ionchannel, PMMA, Planar patch clamp, TRPV1",

author = "Tsuneo Urisu and Hidetaka Uno and Wang, {Zhi Hong} and Obuliraj, {Senthil Kumar} and Noriko Takada and Masaki Aoyama and Mitsukazu Suzui and Toshifumi Asano and Toru Ishizuka and Hiromu Yawo",

note = "Funding Information: Acknowledgements We thank A.M. Rinc{\'o}n for helping with the isolation of the chit33 promoter. M.M.D. was the recipient of a fellowship ``Marina Bueno'' from the Consejo Superior de Investigac-iones Cient{\~o}́ ficas. Work in Sevilla was supported by the grants BIO97-0289 and PL98-4140 from the Comisi{\'o}n Interministerial de Ciencia y Tecnolog{\~o}́ a and the EU respectively and by financial support from the Junta de Andaluc{\~o}́ a. Work in Vienna was supported by a grant from the Austrian Science Foundation (P-13170 MOB).; International Conference on Biomedical Electronics and Devices, BIODEVICES 2012 ; Conference date: 01-02-2012 Through 04-02-2012",

year = "2012",

language = "English",

isbn = "9789898425911",

series = "BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices",

pages = "143--148",

booktitle = "BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices",

}

TY - GEN

T1 - Incubation type planar patch clamp biosensor

T2 - International Conference on Biomedical Electronics and Devices, BIODEVICES 2012

AU - Urisu, Tsuneo

AU - Uno, Hidetaka

AU - Wang, Zhi Hong

AU - Obuliraj, Senthil Kumar

AU - Takada, Noriko

AU - Aoyama, Masaki

AU - Suzui, Mitsukazu

AU - Asano, Toshifumi

AU - Ishizuka, Toru

AU - Yawo, Hiromu

N1 - Funding Information: Acknowledgements We thank A.M. Rincón for helping with the isolation of the chit33 promoter. M.M.D. was the recipient of a fellowship ``Marina Bueno'' from the Consejo Superior de Investigac-iones Cientṍ ficas. Work in Sevilla was supported by the grants BIO97-0289 and PL98-4140 from the Comisión Interministerial de Ciencia y Tecnologṍ a and the EU respectively and by financial support from the Junta de Andalucṍ a. Work in Vienna was supported by a grant from the Austrian Science Foundation (P-13170 MOB).

PY - 2012

Y1 - 2012

N2 - The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.

AB - The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.

KW - Biosensor

KW - HEK293

KW - Ionchannel

KW - PMMA

KW - Planar patch clamp

KW - TRPV1

UR - http://www.scopus.com/inward/record.url?scp=84861960892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861960892&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84861960892

SN - 9789898425911

T3 - BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

SP - 143

EP - 148

BT - BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

Y2 - 1 February 2012 through 4 February 2012

ER -

Incubation type planar patch clamp biosensor: Basic performances

Abstract

Publication series

Other

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this