Induction of cross‐fertilization between sea urchin eggs and starfish sperm by polyethylene glycol treatment

Keiichiro Kyozuka; Kenzi Osanai

doi:10.1002/mrd.1120220202

Induction of cross‐fertilization between sea urchin eggs and starfish sperm by polyethylene glycol treatment

Keiichiro Kyozuka, Kenzi Osanai

LIF - Research Center for Marine Biology, Asamushi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Cross‐fertilization between sea urchin eggs (Strongylocentrotus nudus) and starfish sperm (Asterina pectinifera) was induced by treatment with polyethylene glycol (PEG). Without treatment with PEG, the denuded egg surface (jelly coat‐ and vitelline coat‐free) engulfed the head of acrosome‐reacted sperm; however, sperm penetration did not occur [Kyozuka and Osanai, 1988]. When these eggs were exposed briefly to PEG (molecular weight 3,000) in seawater, the sperm entered the egg by membrane fusion. Cortical granules were discharged, and embryogenesis began following sperm penetration. PEG did not induce parthenogenesis in Strongylocentrotus eggs. Egg activation is thus closely linked with gamete membrane fusion.

Original language	English
Pages (from-to)	123-129
Number of pages	7
Journal	Gamete Research
Volume	22
Issue number	2
DOIs	https://doi.org/10.1002/mrd.1120220202
Publication status	Published - 1989 Feb

Keywords

acrosome reaction
gamete membrane fusion
interclass crossing
sperm engulfment

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1002/mrd.1120220202

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title = "Induction of cross‐fertilization between sea urchin eggs and starfish sperm by polyethylene glycol treatment",

abstract = "Cross‐fertilization between sea urchin eggs (Strongylocentrotus nudus) and starfish sperm (Asterina pectinifera) was induced by treatment with polyethylene glycol (PEG). Without treatment with PEG, the denuded egg surface (jelly coat‐ and vitelline coat‐free) engulfed the head of acrosome‐reacted sperm; however, sperm penetration did not occur [Kyozuka and Osanai, 1988]. When these eggs were exposed briefly to PEG (molecular weight 3,000) in seawater, the sperm entered the egg by membrane fusion. Cortical granules were discharged, and embryogenesis began following sperm penetration. PEG did not induce parthenogenesis in Strongylocentrotus eggs. Egg activation is thus closely linked with gamete membrane fusion.",

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author = "Keiichiro Kyozuka and Kenzi Osanai",

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T1 - Induction of cross‐fertilization between sea urchin eggs and starfish sperm by polyethylene glycol treatment

AU - Kyozuka, Keiichiro

AU - Osanai, Kenzi

PY - 1989/2

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N2 - Cross‐fertilization between sea urchin eggs (Strongylocentrotus nudus) and starfish sperm (Asterina pectinifera) was induced by treatment with polyethylene glycol (PEG). Without treatment with PEG, the denuded egg surface (jelly coat‐ and vitelline coat‐free) engulfed the head of acrosome‐reacted sperm; however, sperm penetration did not occur [Kyozuka and Osanai, 1988]. When these eggs were exposed briefly to PEG (molecular weight 3,000) in seawater, the sperm entered the egg by membrane fusion. Cortical granules were discharged, and embryogenesis began following sperm penetration. PEG did not induce parthenogenesis in Strongylocentrotus eggs. Egg activation is thus closely linked with gamete membrane fusion.

AB - Cross‐fertilization between sea urchin eggs (Strongylocentrotus nudus) and starfish sperm (Asterina pectinifera) was induced by treatment with polyethylene glycol (PEG). Without treatment with PEG, the denuded egg surface (jelly coat‐ and vitelline coat‐free) engulfed the head of acrosome‐reacted sperm; however, sperm penetration did not occur [Kyozuka and Osanai, 1988]. When these eggs were exposed briefly to PEG (molecular weight 3,000) in seawater, the sperm entered the egg by membrane fusion. Cortical granules were discharged, and embryogenesis began following sperm penetration. PEG did not induce parthenogenesis in Strongylocentrotus eggs. Egg activation is thus closely linked with gamete membrane fusion.

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KW - gamete membrane fusion

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KW - sperm engulfment

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Induction of cross‐fertilization between sea urchin eggs and starfish sperm by polyethylene glycol treatment

Abstract

Keywords

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