TY - JOUR
T1 - Induction of glycophorin gene expression in cultured murine erythroleukemia cells
AU - Matsui, Yasuhisa
AU - Natori, Shunji
AU - Obinata, Masuo
N1 - Funding Information:
Fig. 7. Appearance of glycophorin-positive cells and hemoglobin-positive cells in MEL cells during treatment with DMSO. Cells were stained with antimouse globin antiserum or with antiglyco-phorin antiserum. The percentage of hemoglobin-positive cells (41%) reached its maximum (staining with antiserum) on day 6, while that (11%) of glycophorin reached its maximum on day 7. 0, antiglycophorin; m, antihemoglobin Fig. 8. Induction of glycophorin mRNA in MEL cells. Total RNA was isolated from uninduced (lanes 3, 4) and induced MEL cells (B8/3; fanes 5,6), translated in vitro, and immunoprecipitated with antiserum (lanes 3, 5) or normal serum (Innes 4, 6). As references, the translation products of spleen erythroblasts were immunoprecipitated with antiserum (fane 1) or normal serum (lane 2). The gels were exposed for autoradiography for 30 days Acknowledgements. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.
PY - 1985
Y1 - 1985
N2 - In order to identify the mRNA for mouse glycophorin, mRNA was isolated from immature erythroid cells obtained from the spleens of anemic mice, translated in vitro in mRNA-dependent rabbit reticulocyte lysate, and then immunoprecipitated with a specific antiserum. Glycophorin mRNA was shown to be present only in erythroid cells. In immunofluorescent and in vitro translation studies, it was shown that glycophorin mRNA is absent in uninduced murine erythroleukemia (MEL) cells, but is induced in dimethylsulfoxide-treated differentiating cells.
AB - In order to identify the mRNA for mouse glycophorin, mRNA was isolated from immature erythroid cells obtained from the spleens of anemic mice, translated in vitro in mRNA-dependent rabbit reticulocyte lysate, and then immunoprecipitated with a specific antiserum. Glycophorin mRNA was shown to be present only in erythroid cells. In immunofluorescent and in vitro translation studies, it was shown that glycophorin mRNA is absent in uninduced murine erythroleukemia (MEL) cells, but is induced in dimethylsulfoxide-treated differentiating cells.
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U2 - 10.1111/j.1432-0436.1985.tb00326.x
DO - 10.1111/j.1432-0436.1985.tb00326.x
M3 - Article
C2 - 3908202
AN - SCOPUS:0022233907
SN - 0301-4681
VL - 29
SP - 268
EP - 274
JO - Differentiation
JF - Differentiation
IS - 3
ER -
