TY - JOUR
T1 - Induction of IFN-γ and IL-1α production in macrophages stimulated with phosphopolysaccharide produced by Lactococcus lactis ssp. cremoris
AU - Kitazawa, Haruki
AU - Itoh, Takatoshi
AU - Tomioka, Yoshihisa
AU - Mizugaki, Michinao
AU - Yamaguchi, Takahiro
N1 - Funding Information:
We are grateful to Dr. V. K. Singh for his critical readingo f the manuscript. This work was partially supportedb y a Grant-in-Aid for ScientificR esearch( C) (Number 06660331)t o Dr. T. Itoh and a Grant-in-Aid for encouragemenot f young scientists( Number 04760193t)o Dr. H. Kitazawa from the Ministry of Education, Sciencea nd Culture of Japan.
PY - 1996/8
Y1 - 1996/8
N2 - The induction of interferon (IFN) and interleukin-1 (IL-1) production in murine macrophages by a phosphopolysaccharide, produced by a dairy lactic acid bacteria, Lactococcus lactis ssp. cremoris, was investigated. When the phosphopolysaccharide was added into macrophage cultures at concentrations from 1 to 200 μg/ml, substantial IFN titers (6.2-79.2 IU/ml) were detected. Using the reverse transcription-polymerase chain reaction (RT-PCR), the expression of mRNA encoding IFN-γ was verified in spleen macrophage cultures. Macrophages stimulated with the phosphopolysaccharide also produced IL-1α at a concentration of 50 μg/ml. This study showed for the first time that phosphopolysaccharide derived from a dairy lactic acid bacterium can induce IFN-γ and IL-1α production in macrophages. These findings strongly suggest that the phosphopolysaccharide is a type of 'biological response modifier' and the fermented dairy foods containing Lactococcus lactis ssp. cremoris can be designated as a physiologically functional food.
AB - The induction of interferon (IFN) and interleukin-1 (IL-1) production in murine macrophages by a phosphopolysaccharide, produced by a dairy lactic acid bacteria, Lactococcus lactis ssp. cremoris, was investigated. When the phosphopolysaccharide was added into macrophage cultures at concentrations from 1 to 200 μg/ml, substantial IFN titers (6.2-79.2 IU/ml) were detected. Using the reverse transcription-polymerase chain reaction (RT-PCR), the expression of mRNA encoding IFN-γ was verified in spleen macrophage cultures. Macrophages stimulated with the phosphopolysaccharide also produced IL-1α at a concentration of 50 μg/ml. This study showed for the first time that phosphopolysaccharide derived from a dairy lactic acid bacterium can induce IFN-γ and IL-1α production in macrophages. These findings strongly suggest that the phosphopolysaccharide is a type of 'biological response modifier' and the fermented dairy foods containing Lactococcus lactis ssp. cremoris can be designated as a physiologically functional food.
KW - IFN-γ
KW - IL-1α mRNA expression
KW - Lactococcus lactis ssp. cremoris
KW - Murine macrophage
KW - Phosphopolysaccharide
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U2 - 10.1016/0168-1605(96)00968-3
DO - 10.1016/0168-1605(96)00968-3
M3 - Article
C2 - 8880300
AN - SCOPUS:0030220946
SN - 0168-1605
VL - 31
SP - 99
EP - 106
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 1-3
ER -