TY - JOUR
T1 - Influence of cellular shape on sliding behavior of ciliates
AU - Nishigami, Yukinori
AU - Ohmura, Takuya
AU - Taniguchi, Atsushi
AU - Nonaka, Shigenori
AU - Manabe, Junichi
AU - Ishikawa, Takuji
AU - Ichikawa, Masatoshi
N1 - Funding Information:
The Paramecium cells used in this study were provided by the Symbiosis Laboratory at Yamaguchi University with support from the National Bio-Resource Project of the Japan Agency for Medical Research and Development.
Funding Information:
Paramecium caudatum (Ai253) was purchased from the Symbiosis Laboratory, Yamaguchi University, with support from the National Bio-Resource Project (NBRP) of the Japan Agency for Medical Research and Development (NBRP-Paramecium, http://nbrpcms.nig. ac.jp/paramecium/). The cells were cultured in a barley grass solution (0.3 g/ml Young barley grass power (Yamamoto kanpoh Pharmacetical) dissolved in distilled water) bacterized with Enterobacter aerogenes at 25°C. Before observation, the cells in mid-log phase were washed three times with an observation solution (1.4 mM Na2HPO4, 0.6 mM NaH2PO4, 2 mM NaC6H5O7 citrate, and 1.5 mM CaCl2).
Funding Information:
This work was supported by the Japan Society for the Promotion of Science KAKENHI Grants JP17J06827, JP17J10331, JP26707020, and JP25103012, NIBB Collaborative Research Program (18-503) to M.I. and promoted by the Mathematics-based Creation of Science (MACS) program, Kyoto University.
Publisher Copyright:
© 2018, © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2018/7/4
Y1 - 2018/7/4
N2 - Some types of ciliates accumulate on solid/fluid interfaces. This behavior is advantageous to survival in nature due to the presence of sufficient nutrition and stable environments. Recently, the accumulating mechanisms of Tetrahymena pyriformis at the interface were investigated. The synergy of the ellipsoidal shape of the cell body and the mechanosensing feature of the cilia allow for cells to slide on interfaces, and the sliding behavior leads to cell accumulation on the interfaces. Here, to examine the generality of the sliding behavior of ciliates, we characterized the behavior of Paramecium caudatum, which is a commonly studied ciliate. Our experimental and numerical results confirmed that P. caudatum also slid on the solid/fluid interface by using the same mechanism as T. pyriformis. In addition, we evaluated the effects of cellular ellipticity on their behaviors near the wall with a phase diagram produced via numerical simulation.
AB - Some types of ciliates accumulate on solid/fluid interfaces. This behavior is advantageous to survival in nature due to the presence of sufficient nutrition and stable environments. Recently, the accumulating mechanisms of Tetrahymena pyriformis at the interface were investigated. The synergy of the ellipsoidal shape of the cell body and the mechanosensing feature of the cilia allow for cells to slide on interfaces, and the sliding behavior leads to cell accumulation on the interfaces. Here, to examine the generality of the sliding behavior of ciliates, we characterized the behavior of Paramecium caudatum, which is a commonly studied ciliate. Our experimental and numerical results confirmed that P. caudatum also slid on the solid/fluid interface by using the same mechanism as T. pyriformis. In addition, we evaluated the effects of cellular ellipticity on their behaviors near the wall with a phase diagram produced via numerical simulation.
KW - Paramecium caudatum
KW - Sliding on the wall
KW - ciliates
KW - unicellular behaviors
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U2 - 10.1080/19420889.2018.1506666
DO - 10.1080/19420889.2018.1506666
M3 - Article
AN - SCOPUS:85052120987
SN - 1942-0889
VL - 11
JO - Communicative and Integrative Biology
JF - Communicative and Integrative Biology
IS - 4
M1 - e1506666
ER -