Influence of immediate and early loading on bone metabolic activity around dental implants in rat tibiae

Miou Yamamoto, Toru Ogawa, Masayoshi Yokoyama, Shigeto Koyama, Keiichi Sasaki

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Objectives: The aim of this study was to examine the influence of immediate and early loading on dynamic changes in bone metabolism around dental implants using bone scintigraphy. Material and methods: Two titanium implants were inserted in the right tibiae of 21 rats. Closed coil springs with 4.0-N loads were applied parallel to the upper portion of the implants for 35 days. According to the load application timing, rats were divided into three groups: immediate loading (IL) group, early loading 1 day after implant insertion (1-D early loading [EL]) group, and loading 3 days after implant insertion (3-D EL) group. Rats were intravenously injected with technetium-99 m-methylene diphosphonate (Tc99 m-MDP) (74 MBq/rat) and scanned by bone scintigraphy at 1, 4, 7, 11, 14, 21, 28, and 35 days after load application. The ratio of accumulation of Tc99 m-MDP around the implants to that of a reference site (uptake ratio) was calculated to evaluate bone metabolism. Results: In every group, the uptake ratio increased until 7 days after load application and then gradually decreased. It was significantly higher than baseline at 4, 7, 11, and 14 days (P < 0.001). The uptake ratio in the 1-D EL and 3-D EL groups were significantly higher than that in the control group and also that in the IL group (P < 0.001). Conclusions: Bone metabolism initially increased and then gradually decreased to baseline despite differences in load timing. Increases in bone metabolic activity differed according to load application timing; the later the load application, the more enhanced the bone metabolism.

Original languageEnglish
Pages (from-to)1084-1090
Number of pages7
JournalClinical Oral Implants Research
Issue number9
Publication statusPublished - 2014 Sept


  • Bone metabolic activity
  • Dental implant
  • Early loading
  • Immediate loading
  • Peri-implant bone
  • Rat tibia


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