TY - JOUR
T1 - Influence of osmotic and cationic stresses on high pressure inactivation of Escherichia coli
AU - Hasegawa, Toshimi
AU - Nakamura, Takuya
AU - Hayashi, Mayumi
AU - Kido, Miyuki
AU - Hirayama, Masao
AU - Yamaguchi, Toshio
AU - Iguchi, Akinori
AU - Ueno, Shigeaki
AU - Shigematsu, Toru
AU - Fujii, Tomoyuki
N1 - Funding Information:
This work was supported by the Niigata Prefecture Collaboration of Regional Entities for the Advancement of Technological Excellence program of the Japan Science and Technology Agency and by the Salt Science Research Foundation.
PY - 2013/6/1
Y1 - 2013/6/1
N2 - We analyzed high hydrostatic pressure (HP)-induced inactivation of Escherichia coli in the presence of sucrose, NaCl, KCl, or LiCl under 250-400 MPa. Salt stress response (osmotic or cationic) mechanism was demonstrated to be evaluated by HP-induced inactivation kinetics. We selected nine mutant strains from the Keio collection; five strains each lacked one osmoregulatory gene (ΔmzrA, ΔenvZ, ΔompR, ΔompC or ΔompF), and four strains each lacked one cationic stress-response gene (ΔmscS, ΔmscL, ΔchaA or ΔnhaA). The nine mutants exhibited three different patterns of HP-induced inactivation. Of the nine genes, four (mzrA, mscS, nhaA and mscL) were involved in piezotolerance at NaCl concentration of 0.0-0.35 M.
AB - We analyzed high hydrostatic pressure (HP)-induced inactivation of Escherichia coli in the presence of sucrose, NaCl, KCl, or LiCl under 250-400 MPa. Salt stress response (osmotic or cationic) mechanism was demonstrated to be evaluated by HP-induced inactivation kinetics. We selected nine mutant strains from the Keio collection; five strains each lacked one osmoregulatory gene (ΔmzrA, ΔenvZ, ΔompR, ΔompC or ΔompF), and four strains each lacked one cationic stress-response gene (ΔmscS, ΔmscL, ΔchaA or ΔnhaA). The nine mutants exhibited three different patterns of HP-induced inactivation. Of the nine genes, four (mzrA, mscS, nhaA and mscL) were involved in piezotolerance at NaCl concentration of 0.0-0.35 M.
KW - Escherichia coli
KW - Keio collection
KW - high hydrostatic pressure
KW - osmotic and cationic stress
KW - piezotolerance
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U2 - 10.1080/08957959.2013.800513
DO - 10.1080/08957959.2013.800513
M3 - Article
AN - SCOPUS:84881226674
SN - 0895-7959
VL - 33
SP - 292
EP - 298
JO - High Pressure Research
JF - High Pressure Research
IS - 2
ER -