Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy

Kuniaki Nagamine; Yasufumi Takahashi; Kosuke Ino; Hitoshi Shiku; Tomokazu Matsue

doi:10.1002/elan.201000595

Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy

Kuniaki Nagamine, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in μm-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.

Original language	English
Pages (from-to)	1168-1174
Number of pages	7
Journal	Electroanalysis
Volume	23
Issue number	5
DOIs	https://doi.org/10.1002/elan.201000595
Publication status	Published - 2011 May

Keywords

Nanoelectrodes
Scanning electrochemical microscopy
Single cell analysis

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

Access to Document

10.1002/elan.201000595

Cite this

@article{58081667641444a3a452b14f4c752795,

title = "Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy",

abstract = "The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in μm-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.",

keywords = "Nanoelectrodes, Scanning electrochemical microscopy, Single cell analysis",

author = "Kuniaki Nagamine and Yasufumi Takahashi and Kosuke Ino and Hitoshi Shiku and Tomokazu Matsue",

year = "2011",

month = may,

doi = "10.1002/elan.201000595",

language = "English",

volume = "23",

pages = "1168--1174",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

number = "5",

}

TY - JOUR

T1 - Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy

AU - Nagamine, Kuniaki

AU - Takahashi, Yasufumi

AU - Ino, Kosuke

AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

PY - 2011/5

Y1 - 2011/5

N2 - The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in μm-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.

AB - The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in μm-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.

KW - Nanoelectrodes

KW - Scanning electrochemical microscopy

KW - Single cell analysis

UR - http://www.scopus.com/inward/record.url?scp=79955579302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955579302&partnerID=8YFLogxK

U2 - 10.1002/elan.201000595

DO - 10.1002/elan.201000595

M3 - Article

AN - SCOPUS:79955579302

SN - 1040-0397

VL - 23

SP - 1168

EP - 1174

JO - Electroanalysis

JF - Electroanalysis

IS - 5

ER -

Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this