Influenza virus utilizes N-linked sialoglycans as receptors in A549 cells

Kazuya I.P.J. Hidari, Maho Yamaguchi, Fumihiko Ueno, Tomoko Abe, Kazuhiro Yoshida, Takashi Suzuki

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Influenza viruses (IFVs) recognize sialoglycans expressed on the host cell surface. To understand the mechanisms underlying tissue and host tropisms of IFV, it is essential to elucidate the molecular interaction of the virus with the host sialoglycan receptor. We established and applied a new monoclonal antibody, clone HYB4, which specifically recognizes the Neu5Acα2-3 determinant at the non-reducing terminal Gal residue of both glycoproteins and gangliosides to investigate the biochemical properties of IFV receptors in A549 cells. HYB4 significantly blocked virus binding to A549 cells in a dose-dependent manner. Virus overlay assay indicated that several glycoproteins with molecular masses of 80-120kDa of A549 cells were commonly recognized by different subtypes of IFV, such as H1N1 and H3N2. H1N1 virus binding to the glycoproteins was diminished by pretreatment with either sialidase or PNGase F. On TLC-immunostaining experiments with HYB4, GM3 ganglioside was only detected in A549 cells. Interestingly, this antibody bound to GM3 gangliosides on TLC and plastic surfaces, but not on lipid bilayers. In comparison with the recognition of Maackia amurensis lectins, HYB4 exclusively recognized Neu5Acα2-3Galβ1-4GlcNAc residues expressed on glycoproteins. These results strongly suggest that N-linked sialoglycans with the Neu5Acα2-3 determinant on several glycoproteins are receptors for influenza virus in A549 cells.

Original languageEnglish
Pages (from-to)394-399
Number of pages6
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - 2013 Jul 5
Externally publishedYes


  • Influenza virus
  • Monoclonal antibody
  • Receptor
  • Siaα2-3 glycans

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Influenza virus utilizes N-linked sialoglycans as receptors in A549 cells'. Together they form a unique fingerprint.

Cite this