TY - JOUR
T1 - Infusion of oxytocin induces successful delivery in prostanoid FP-receptor-deficient mice
AU - Kawamata, Masaki
AU - Yoshida, Masahide
AU - Sugimoto, Yukihiko
AU - Kimura, Tadashi
AU - Tonomura, Yutaka
AU - Takayanagi, Yuki
AU - Yanagisawa, Teruyuki
AU - Nishimori, Katsuhiko
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research (No. 14360046) from the Ministry of Education, Science and Culture of Japan and by Sankyo Foundation of Life Science.
PY - 2008/2/13
Y1 - 2008/2/13
N2 - The dramatic increase of oxytocin (OT) receptor (OTR) in the myometrium as well as circulating progesterone withdrawal has been thought to be the most important factor in the induction and accomplishment of parturition since delivery fails in prostaglandin F2α receptor (FP) knockout (FP KO) mice. The expression levels of OTR mRNA/protein were not dramatically increased in the near-term uteri of FP KO mice. However, OT-induced myometrial contractions and the concentration-response curves in FP KO in vitro were almost similar to those in wild-type (WT) mice. OT-infusion (0.3 U/day) enabled FP KO mice to experience successful delivery, and furthermore the duration until the onset was hastened by a higher dose of OT (3 U/day). The plasma progesterone levels of FP KO females were maintained at high levels, but decreased during labor by OT-infusion (3 U/day). These results suggest that OT has potentials to induce strong myometrial contractions in uterus with low expression levels of OTR and luteolysis in ovary, which enabled FP KO females to undergo successful delivery.
AB - The dramatic increase of oxytocin (OT) receptor (OTR) in the myometrium as well as circulating progesterone withdrawal has been thought to be the most important factor in the induction and accomplishment of parturition since delivery fails in prostaglandin F2α receptor (FP) knockout (FP KO) mice. The expression levels of OTR mRNA/protein were not dramatically increased in the near-term uteri of FP KO mice. However, OT-induced myometrial contractions and the concentration-response curves in FP KO in vitro were almost similar to those in wild-type (WT) mice. OT-infusion (0.3 U/day) enabled FP KO mice to experience successful delivery, and furthermore the duration until the onset was hastened by a higher dose of OT (3 U/day). The plasma progesterone levels of FP KO females were maintained at high levels, but decreased during labor by OT-infusion (3 U/day). These results suggest that OT has potentials to induce strong myometrial contractions in uterus with low expression levels of OTR and luteolysis in ovary, which enabled FP KO females to undergo successful delivery.
KW - FP
KW - Knockout mice
KW - Oxytocin
KW - Oxytocin receptor
KW - Parturition
KW - Uterus
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U2 - 10.1016/j.mce.2007.10.012
DO - 10.1016/j.mce.2007.10.012
M3 - Article
C2 - 18083301
AN - SCOPUS:39149108429
SN - 0303-7207
VL - 283
SP - 32
EP - 37
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -