TY - JOUR
T1 - Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japonicus
AU - Sugaya, Takuma
AU - Ikeda, Minoru
AU - Mori, Hideshi
AU - Taniguchi, Nobuhiko
PY - 2002
Y1 - 2002
N2 - The allelic inheritance mode of microsatellite DNA markers was examined using seven copulated wild females and their offspring. Five microsatellite loci, CSPJ002*, CSPJ010*, CSPJ012*, CSPJ014*, and CSPJ015*, were used in the study. At almost all family/locus combinations, one sire was determined and distributions of genotypes in offspring were consistent with the Mendelian segregation ratio. Distributions of genotypes were consistent with the ratio after assuming a null allele at some loci. Consequently, the alleles of CSPJ002* and CSPJ012* were inherited following the Mendelian inheritance mode in every family; however, the null allele was expected in CSPJ010*, CSPJ014*, and CSPJ015* in some families. Thus, these loci should be used carefully in population genetic analysis, but siblings could be detected in the dendrograms based on unweighted pair-group method using arithmetic averages (UPMGA).
AB - The allelic inheritance mode of microsatellite DNA markers was examined using seven copulated wild females and their offspring. Five microsatellite loci, CSPJ002*, CSPJ010*, CSPJ012*, CSPJ014*, and CSPJ015*, were used in the study. At almost all family/locus combinations, one sire was determined and distributions of genotypes in offspring were consistent with the Mendelian segregation ratio. Distributions of genotypes were consistent with the ratio after assuming a null allele at some loci. Consequently, the alleles of CSPJ002* and CSPJ012* were inherited following the Mendelian inheritance mode in every family; however, the null allele was expected in CSPJ010*, CSPJ014*, and CSPJ015* in some families. Thus, these loci should be used carefully in population genetic analysis, but siblings could be detected in the dendrograms based on unweighted pair-group method using arithmetic averages (UPMGA).
KW - Allelic inheritance
KW - Kinship
KW - Microsatellite DNA
KW - Null allele
KW - Penaeus japonicus
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U2 - 10.1046/j.1444-2906.2002.00425.x
DO - 10.1046/j.1444-2906.2002.00425.x
M3 - Article
AN - SCOPUS:0036091445
SN - 0919-9268
VL - 68
SP - 299
EP - 305
JO - Fisheries Science
JF - Fisheries Science
IS - 2
ER -