Inhibition by dexamethasone of interleukin 13 production via glucocorticoid receptor-mediated inhibition of c-Jun phosphorylation

Noriyasu Hirasawa; Shinichiroh Izumi; Watchara Linwong; Kazuo Ohuchi

doi:10.1016/S0014-5793(03)01228-6

Inhibition by dexamethasone of interleukin 13 production via glucocorticoid receptor-mediated inhibition of c-Jun phosphorylation

Noriyasu Hirasawa, Shinichiroh Izumi, Watchara Linwong, Kazuo Ohuchi

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The antigen stimulation of RBL-2H3 cells induced interleukin 13 (IL-13) production, which was inhibited by the steroidal anti-inflammatory drug dexamethasone and by the c-Jun N-terminal kinase (JNK) inhibitor SP600125. Dexamethasone did not inhibit the antigen-induced phosphorylation of JNK but inhibited that of c-Jun. In a cell-free system, the phosphorylation of glutathione S-transferase-fused c-Jun by recombinant JNK was not inhibited by dexamethasone but was inhibited by the addition of recombinant glucocorticoid receptor (GR). These findings suggest that the inhibition of antigen-induced IL-13 production by dexamethasone is due to the GR-mediated inhibition of c-Jun phosphorylation induced by JNK.

Original language	English
Pages (from-to)	489-493
Number of pages	5
Journal	FEBS Letters
Volume	554
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(03)01228-6
Publication status	Published - 2003 Nov 20

Keywords

Dexamethasone
Glucocorticoid receptor
Interleukin 13
RBL-2H3 cell line
c-Jun
c-Jun N-terminal kinase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(03)01228-6

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title = "Inhibition by dexamethasone of interleukin 13 production via glucocorticoid receptor-mediated inhibition of c-Jun phosphorylation",

abstract = "The antigen stimulation of RBL-2H3 cells induced interleukin 13 (IL-13) production, which was inhibited by the steroidal anti-inflammatory drug dexamethasone and by the c-Jun N-terminal kinase (JNK) inhibitor SP600125. Dexamethasone did not inhibit the antigen-induced phosphorylation of JNK but inhibited that of c-Jun. In a cell-free system, the phosphorylation of glutathione S-transferase-fused c-Jun by recombinant JNK was not inhibited by dexamethasone but was inhibited by the addition of recombinant glucocorticoid receptor (GR). These findings suggest that the inhibition of antigen-induced IL-13 production by dexamethasone is due to the GR-mediated inhibition of c-Jun phosphorylation induced by JNK.",

keywords = "Dexamethasone, Glucocorticoid receptor, Interleukin 13, RBL-2H3 cell line, c-Jun, c-Jun N-terminal kinase",

author = "Noriyasu Hirasawa and Shinichiroh Izumi and Watchara Linwong and Kazuo Ohuchi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Specific Research on Priority Areas (12139202) from the Ministry of Education, Science, Sports and Culture of Japan.",

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T1 - Inhibition by dexamethasone of interleukin 13 production via glucocorticoid receptor-mediated inhibition of c-Jun phosphorylation

AU - Hirasawa, Noriyasu

AU - Izumi, Shinichiroh

AU - Linwong, Watchara

AU - Ohuchi, Kazuo

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Specific Research on Priority Areas (12139202) from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 2003/11/20

Y1 - 2003/11/20

N2 - The antigen stimulation of RBL-2H3 cells induced interleukin 13 (IL-13) production, which was inhibited by the steroidal anti-inflammatory drug dexamethasone and by the c-Jun N-terminal kinase (JNK) inhibitor SP600125. Dexamethasone did not inhibit the antigen-induced phosphorylation of JNK but inhibited that of c-Jun. In a cell-free system, the phosphorylation of glutathione S-transferase-fused c-Jun by recombinant JNK was not inhibited by dexamethasone but was inhibited by the addition of recombinant glucocorticoid receptor (GR). These findings suggest that the inhibition of antigen-induced IL-13 production by dexamethasone is due to the GR-mediated inhibition of c-Jun phosphorylation induced by JNK.

AB - The antigen stimulation of RBL-2H3 cells induced interleukin 13 (IL-13) production, which was inhibited by the steroidal anti-inflammatory drug dexamethasone and by the c-Jun N-terminal kinase (JNK) inhibitor SP600125. Dexamethasone did not inhibit the antigen-induced phosphorylation of JNK but inhibited that of c-Jun. In a cell-free system, the phosphorylation of glutathione S-transferase-fused c-Jun by recombinant JNK was not inhibited by dexamethasone but was inhibited by the addition of recombinant glucocorticoid receptor (GR). These findings suggest that the inhibition of antigen-induced IL-13 production by dexamethasone is due to the GR-mediated inhibition of c-Jun phosphorylation induced by JNK.

KW - Dexamethasone

KW - Glucocorticoid receptor

KW - Interleukin 13

KW - RBL-2H3 cell line

KW - c-Jun

KW - c-Jun N-terminal kinase

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U2 - 10.1016/S0014-5793(03)01228-6

DO - 10.1016/S0014-5793(03)01228-6

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C2 - 14623117

AN - SCOPUS:0242626519

SN - 0014-5793

VL - 554

SP - 489

EP - 493

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Inhibition by dexamethasone of interleukin 13 production via glucocorticoid receptor-mediated inhibition of c-Jun phosphorylation

Abstract

Keywords

ASJC Scopus subject areas

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