TY - JOUR
T1 - Inhibition by gossypol of tumor promoter-induced arachidonic acid metabolism in rat peritoneal macrophages
AU - Ohuchi, Kazuo
AU - Watanabe, Masako
AU - Hirasawa, Noriyasu
AU - Tsurufuji, Susumu
AU - Ozeki, Tsuneo
AU - Fujiki, Hirota
PY - 1988/8/19
Y1 - 1988/8/19
N2 - Rat peritoneal macrophages were prelabeled with [3H]arachidonic acid. The release of radioactivity into the medium was increased by treatment with TPA-type tumor promoters, such as TPA, teleocidin and aplysiatoxin, and the non-TPA-type tumor promoter, thapsigargin. Gossypol, at concentrations of 3 and 10 μM, inhibited the release of radioactivity stimulated by both types of tumor promoter, although the mechanism of stimulation of arachidonic acid metabolism is different in the two types of tumor promoter. Stimulation of prostaglandin E2 production by these tumor promoters was also inhibited by treatment with gossypol. Calcium ionophore A23187-stimulated release of radioactivity and prostaglandin E2 production were also inhibited by gossypol treatment. The mechanism of inhibition by gossypol of prostaglandin E2 production is discussed.
AB - Rat peritoneal macrophages were prelabeled with [3H]arachidonic acid. The release of radioactivity into the medium was increased by treatment with TPA-type tumor promoters, such as TPA, teleocidin and aplysiatoxin, and the non-TPA-type tumor promoter, thapsigargin. Gossypol, at concentrations of 3 and 10 μM, inhibited the release of radioactivity stimulated by both types of tumor promoter, although the mechanism of stimulation of arachidonic acid metabolism is different in the two types of tumor promoter. Stimulation of prostaglandin E2 production by these tumor promoters was also inhibited by treatment with gossypol. Calcium ionophore A23187-stimulated release of radioactivity and prostaglandin E2 production were also inhibited by gossypol treatment. The mechanism of inhibition by gossypol of prostaglandin E2 production is discussed.
KW - (Rat peritoneal macrophage)
KW - Arachidonic acid metabolism
KW - Gossypol
KW - Prostaglandin E
KW - Tumor promotor
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U2 - 10.1016/0167-4889(88)90164-4
DO - 10.1016/0167-4889(88)90164-4
M3 - Article
C2 - 3136806
AN - SCOPUS:0023730738
SN - 0167-4889
VL - 971
SP - 85
EP - 91
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 1
ER -