Inhibition of tooth demineralization caused by Streptococcus mutans biofilm via antimicrobial treatment using hydrogen peroxide photolysis

Midori Shirato, Keisuke Nakamura, Taichi Tenkumo, Yoshimi Niwano, Taro Kanno, Keiichi Sasaki, Peter Lingström, Ulf Örtengren

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Objectives: An antimicrobial technique utilizing hydroxyl radicals generated by the photolysis of 3% H2O2 has been developed recently. The present study aimed to evaluate the effect of H2O2 photolysis treatment on tooth demineralization caused by Streptococcus mutans biofilm. Materials and methods: To induce tooth demineralization, S. mutans biofilm was allowed to form on the maxillary first molars collected from Wistar rats via 24-h culturing. The samples were immersed in 3% H2O2 and irradiated with 365-nm LED (H2O2 photolysis treatment). Viable bacterial counts in the biofilm were evaluated immediately after treatment and after an additional 30-h culturing by colony counting. The acidogenicity of the biofilm, re-established 30 h after treatment, was assessed by measuring the pH. The effect of H2O2 photolysis treatment on tooth demineralization was assessed by measuring the depth of the radiolucent layer in micro-CT images. Results: H2O2 photolysis significantly reduced viable bacterial counts in the biofilm to 3.7 log colony forming units (CFU)/sample, while the untreated group had 7.9 log CFU/sample. The pH of the biofilm re-established after treatment (6.6) was higher than that of the untreated group (5.3). In line with the pH measurement, the treatment group had a significantly lower depth of radiolucent layer in dentin than the untreated group. Conclusions: H2O2 photolysis treatment was effective not only in killing the biofilm-forming S. mutans but also in lowering the acidogenicity of the biofilm. Thus, this technique could inhibit tooth demineralization. Clinical relevance: H2O2 photolysis can be applicable as a new dental caries treatment.

Original languageEnglish
Pages (from-to)739-750
Number of pages12
JournalClinical Oral Investigations
Volume27
Issue number2
DOIs
Publication statusPublished - 2023 Feb

Keywords

  • Biofilms
  • Demineralization
  • Dental caries
  • Hydrogen peroxide
  • Streptococcus mutans

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